Nunoura Takuro, Miyazaki Masayuki, Suzuki Yohey, Takai Ken, Horikoshi Koki
Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):676-81. doi: 10.1099/ijs.0.64615-0.
A novel extremely thermophilic sulfur-oxidizing bacterium, strain LS12-2(T), was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain LS12-2(T) were motile rods, 1.5-4.0 microm in length and 0.4-0.5 microm in width. Strain LS12-2(T) was an obligate chemolithoautotroph that could utilize elemental sulfur or thiosulfate as an electron donor and nitrate or oxygen as an electron acceptor. Growth was observed at 65-85 degrees C (optimum 70-75 degrees C), pH 5.8-8.3 (optimum pH 6.9-7.5), 1.0-4.0 % (w/v) NaCl (optimum 2.5 %) and 1.0-7.0 % O(2) in the gas phase (optimum 3.0 %). Fatty acids detected were C(16 : 0) (8.0 %), C(18 : 0) (9.0 %), C(18 : 1) (62.5 %) and C(20 : 1) (20.5 %). The genomic DNA G+C content was 51.3 mol%. 16S rRNA gene sequence analysis indicated that strain LS12-2(T) belonged to the genus Hydrogenivirga. Based on physiological and phylogenetic characteristics of the isolate, it is proposed that this strain represents a novel species in the genus Hydrogenivirga, Hydrogenivirga okinawensis sp. nov. The type strain of Hydrogenivirga okinawensis is LS12-2(T) (=JCM 13302(T)=DSM 17378(T)).
从冲绳海槽南部与那国岛海丘四号的深海热液区分离出了一种新型的嗜热硫氧化细菌，菌株LS12-2(T)。菌株LS12-2(T)的细胞为能动杆菌，长1.5 - 4.0微米，宽0.4 - 0.5微米。菌株LS12-2(T)是专性化能无机自养菌，可利用元素硫或硫代硫酸盐作为电子供体，硝酸盐或氧气作为电子受体。在65 - 85℃（最适温度70 - 75℃）、pH 5.8 - 8.3（最适pH 6.9 - 7.5）、1.0 - 4.0%（w/v）NaCl（最适2.5%）和气相中1.0 - 7.0% O₂（最适3.0%）条件下可观察到生长。检测到的脂肪酸为C₁₆:₀（8.0%）、C₁₈:₀（9.0%）、C₁₈:₁（62.5%）和C₂₀:₁（20.5%）。基因组DNA的G+C含量为51.3 mol%。16S rRNA基因序列分析表明菌株LS12-2(T)属于氢弧菌属。基于该分离株的生理和系统发育特征，提议将该菌株代表氢弧菌属中的一个新物种，即冲绳氢弧菌（Hydrogenivirga okinawensis sp. nov.）。冲绳氢弧菌的模式菌株为LS12-2(T)（=JCM 13302(T)=DSM 17378(T)）。
