Edwards Joan E, Kim Eun Joong, Davies David R, Hanafy Radwa, Kingston-Smith Alison H
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan Campus, Aberystwyth SY23 3EE, UK.
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.
Microorganisms. 2023 Sep 25;11(10):2390. doi: 10.3390/microorganisms11102390.
Sampling of ruminant saliva has gained interest as a non-invasive proxy for exploring the structure of the rumen microbiome. However, the subsequent data analysis assumes that bacteria originating from the oral cavity are merely passengers in the rumen and play no active role. In this study, it was hypothesised that metabolically active oral bacteria present in the salivary microbiome play a role in the ruminal degradation of plant material. In vitro cultivation-based enumeration confirmed that the ruminant oral cavity harbours a significant number of anaerobic and cellulolytic bacteria that are metabolically active under ruminal conditions. Bacterial 16S rRNA gene profiling of in vitro enrichments also confirmed that oral-derived bacteria were capable of colonising plant material. Preliminary analysis of the colonising bacteria indicated that bacteria belonging to the genus were of particular interest. In conclusion, the findings of the current study clearly indicate that bolus-associated bacteria have the potential to play a metabolically active role in terms of ruminal colonisation and the degradation of plant material. This evidence confirms the merit of the hypothesis that the metabolically active oral bacteria present in the salivary microbiome may play a role in the ruminal degradation of plant material.
采集反刍动物唾液作为探索瘤胃微生物组结构的一种非侵入性替代方法已引起关注。然而，后续的数据分析假定源自口腔的细菌在瘤胃中只是过客，并无积极作用。在本研究中，我们提出假设，即唾液微生物组中存在的具有代谢活性的口腔细菌在瘤胃中植物材料的降解过程中发挥作用。基于体外培养的计数证实，反刍动物口腔中存在大量在瘤胃条件下具有代谢活性的厌氧和纤维素分解细菌。体外富集物的细菌16S rRNA基因分析也证实，源自口腔的细菌能够在植物材料上定殖。对定殖细菌的初步分析表明，属于[具体属名未给出]属的细菌尤其值得关注。总之，本研究结果清楚地表明，与食团相关的细菌在瘤胃定殖和植物材料降解方面具有发挥代谢活性作用的潜力。这一证据证实了以下假设的价值，即唾液微生物组中存在的具有代谢活性的口腔细菌可能在瘤胃中植物材料的降解过程中发挥作用。
