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参与玫瑰花朵中2-苯乙醇释放的β-葡萄糖苷酶的纯化与表征

Purification and characterization of beta-glucosidase involved in the emission of 2-phenylethanol from rose flowers.

作者信息

Sakai Miwa, Tomita Saori, Hirata Hiroshi, Asai Tatsuo, Dohra Hideo, Hara Masakazu, Watanabe Naoharu

机构信息

The United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Ohya, Shizuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2008 Jan;72(1):219-21. doi: 10.1271/bbb.70404. Epub 2008 Jan 7.

DOI:10.1271/bbb.70404
PMID:18175907
Abstract

Beta-glucosidase was partially purified from Rosa 'Hoh-Jun' petals. The enzyme was highly specific for such beta-D-glucopyranosides as 2-phenylethyl beta-D-glucopyranoside. The optimal activity was observed at pH 6.0 and 35 degrees C. The enzymes were composed with two proteins (160 and 155 kDa) by blue native-PAGE, and were classified in a family 1 glucosidase based on LC-MS/MS analyses.

摘要

β-葡萄糖苷酶从玫瑰‘浩君’花瓣中部分纯化得到。该酶对诸如2-苯乙基β-D-吡喃葡萄糖苷等β-D-吡喃葡萄糖苷具有高度特异性。在pH 6.0和35℃下观察到最佳活性。通过蓝色非变性聚丙烯酰胺凝胶电泳分析，该酶由两种蛋白质（160 kDa和155 kDa）组成，并基于液相色谱-串联质谱分析被归类为1类葡萄糖苷酶。

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