Watanabe Shuzo, Hayashi Kentaro, Yagi Kensuke, Asai Tatsuo, MacTavish Hazel, Picone Joanne, Turnbull Colin, Watanabe Naoharu
The United Graduate School of Agricultural Science, Gifu University, Ohya, Japan.
Biosci Biotechnol Biochem. 2002 May;66(5):943-7. doi: 10.1271/bbb.66.943.
To clarify the biosynthetic pathway to 2-phenylethanol (2), the deuterium-labeled putative precursor, [2H8]L-phenylalanine ([2H8-1]), was fed to the flowers of Rosa 'Hoh-Jun' and R. damascena Mill. throughout maturation, ceasing feeding at the commencement of petal unfurling and at full bloom. Based on GC-MS analyses, [2H8]-1 was incorporated into both 2 and 2-phenylethyl beta-D-glucopyranoside (3) when the flowers were fed until full bloom, whereas no such incorporation into 2 was apparent when feeding was ceased earlier. In both species of rose, the labeling pattern for 2 was almost identical to that for 3, and indicated the presence of [2H6]-, [2H7]- and [2H8]-2, and [2H6]-, [2H7]- and [2H8]-3. This may be ascribed to the equilibrium between 2 and 3. The labeling pattern for 2 and 3 also indicated that these compounds were produced from 1 via several routes, the route involving phenylpyruvic acid being the major one.
为阐明2-苯乙醇（2）的生物合成途径，将氘标记的假定前体[2H8]L-苯丙氨酸（[2H8-1]）施用于‘荷君’玫瑰和大马士革玫瑰的花朵，整个成熟过程均进行施用，在花瓣展开开始时和盛开时停止施用。基于气相色谱-质谱分析，当花朵施用至盛开时，[2H8]-1被整合到2和2-苯乙基β-D-吡喃葡萄糖苷（3）中，而当更早停止施用时，未观察到其整合到2中的现象。在两种玫瑰中，2的标记模式与3几乎相同，表明存在[2H6]-、[2H7]-和[2H8]-2以及[2H6]-、[2H7]-和[2H8]-3。这可能归因于2和3之间的平衡。2和3的标记模式还表明，这些化合物是由1通过几种途径产生的，其中涉及苯丙酮酸的途径是主要途径。
