Université Lyon, Université Saint-Etienne, Centre National de la Recherche Scientifique, Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales, FRE 3727, F-42023 Saint-Etienne, France.
Laboratoire Reproduction et Développement des Plantes, Université Lyon, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, F-69342 Lyon, France.
Plant Physiol. 2019 Mar;179(3):1064-1079. doi: 10.1104/pp.18.01468. Epub 2019 Jan 8.
Floral scent is one of the most important characters in horticultural plants. Roses ( spp.) have been cultivated for their scent since antiquity. However, probably by selecting for cultivars with long vase life, breeders have lost the fragrant character in many modern roses, especially the ones bred for the cut flower market. The genetic inheritance of scent characters has remained elusive so far. In-depth knowledge of this quantitative trait is thus very much needed to breed more fragrant commercial cultivars. Furthermore, rose hybrids harbor a composite genomic structure, which complexifies quantitative trait studies. To understand rose scent inheritance, we characterized a segregating population from two diploid cultivars, × cv H190 and , which have contrasting scent profiles. Several quantitative trait loci for the major volatile compounds in this progeny were identified. One among these loci contributing to the production of 2-phenylethanol, responsible for the characteristic odor of rose, was found to be colocalized with a candidate gene belonging to the 2-phenylethanol biosynthesis pathway: the gene An in-depth allele-specific expression analysis in the progeny demonstrated that only one allele was highly expressed and was responsible for the production of 2-phenylethanol. Unexpectedly, its expression was found to start early during flower development, before the production of the volatile 2-phenylethanol, leading to the accumulation of glycosylated compounds in petals.
花香是园艺植物最重要的特征之一。自古以来，人们就一直因其香味而栽培玫瑰（ spp.）。然而，由于选择具有较长瓶插寿命的品种，许多现代玫瑰，尤其是那些用于切花市场的品种，已经失去了芳香特征。到目前为止，气味特征的遗传遗传仍然难以捉摸。因此，培育更多芳香的商业品种非常需要深入了解这种数量性状。此外，玫瑰杂种具有复杂的基因组结构，这使得数量性状研究更加复杂。为了了解玫瑰香气的遗传，我们对两个二倍体品种 × cv H190 和 的分离群体进行了特征分析，它们具有不同的香气特征。在这个后代中，鉴定出了几个与主要挥发性化合物相关的数量性状基因座。其中一个与 2-苯乙醇产生有关的基因座，负责玫瑰的特征气味，与 2-苯乙醇生物合成途径的候选基因 基因 共定位。在后代中进行的深入等位基因特异性表达分析表明，只有一个等位基因高度表达，负责产生 2-苯乙醇。出乎意料的是，它的表达在挥发性 2-苯乙醇产生之前的花发育早期就开始了，导致花瓣中积累了糖基化化合物。
