Tsukatani Hiroko, Tobiishi Kazuhiro, Tanaka Yoshito, Sakuragi Kenji, Ikeura Tasoh, Nakamura Matayoshi
Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Fukuoka, Japan.
Biosci Biotechnol Biochem. 2008 Jan;72(1):149-54. doi: 10.1271/bbb.70513. Epub 2008 Jan 7.
A simple and selective method was developed for determining the concentration of hexaconazole in river and sea water samples by using liquid chromatography/tandem mass spectrometry with an electrospray ionization interface in the positive ion mode and selective reaction monitoring mode. Trace amounts of hexaconazole were collected in a Sep-Pak Plus tC18 cartridge that was eluted with methanol. The detection limit for hexaconazole was 6 ng/l. The recovery of a standard aqueous solution containing 1 microg/l was 96%. The recovery of hexaconazole in the river and sea water samples was 95% and 90%, respectively. Hexaconazole was not detected in the sea water samples. Trace peaks of hexaconazole were found in the river water samples, the concentration being less than 6 ng/l in all cases. The biological degradation of hexaconazole was tested by using river water. No degradation of hexaconazole was apparent in river water incubated at 20 degrees C for 3 weeks.
建立了一种简单且具有选择性的方法，用于测定河流和海水样品中己唑醇的浓度。该方法采用液相色谱/串联质谱联用技术，配备电喷雾电离接口，以正离子模式和选择性反应监测模式运行。痕量的己唑醇通过Sep-Pak Plus tC18柱收集，并用甲醇洗脱。己唑醇的检测限为6 ng/l。含1 μg/l的标准水溶液的回收率为96%。河流和海水样品中己唑醇的回收率分别为95%和90%。海水中未检测到己唑醇。在河流水样中发现了己唑醇的痕量峰，所有情况下其浓度均低于6 ng/l。采用河水测试了己唑醇的生物降解情况。在20℃下孵育3周的河水中，己唑醇没有明显降解。
