Miyashita Tomoyuki, Hanashita Takuo, Toriyama Michinori, Takagi Ryousuke, Akashika Tamaki, Higashikubo Nobuto
Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, Wakayama, Japan.
Biosci Biotechnol Biochem. 2008 Jan;72(1):37-47. doi: 10.1271/bbb.70302. Epub 2008 Jan 7.
The bone morphogenetic proteins (BMPs) constitute a subfamily of the transforming growth factor type beta (TGF-beta) supergene family. BMP-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. To determine the function of BMP-2 in Pinctada fucata development and hard tissue formation, we isolated a BMP-2 genomic DNA clone and the BMP-2 cDNA. The deduced BMP-2 sequence consisted of 447 amino acids. The BMP-2 gene was composed of three exons. The C-terminal portion (149 amino acids) had 86% and 66% identity to the Crassostrea gigas and the human BMP-2 sequence respectively. The 5' flanking promoter region contained putative glioma transcription factor (Gli) and retinoic acid receptor (RAR) responding elements. The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation.
骨形态发生蛋白（BMPs）构成了转化生长因子β（TGF-β）超基因家族的一个亚家族。BMP-2不仅在成骨细胞分化中起重要作用，而且在发育过程中的模式形成中也起重要作用。为了确定BMP-2在合浦珠母贝发育和硬组织形成中的功能，我们分离了一个BMP-2基因组DNA克隆和BMP-2 cDNA。推导的BMP-2序列由447个氨基酸组成。BMP-2基因由三个外显子组成。C末端部分（149个氨基酸）与太平洋牡蛎和人类BMP-2序列分别具有86%和66%的同源性。5'侧翼启动子区域包含假定的胶质瘤转录因子（Gli）和视黄酸受体（RAR）应答元件。BMP-2基因在套膜组织内部强烈表达，对应于珍珠质文石壳层。这一发现表明BMP-2在珍珠质层形成中起关键作用。
