Hung Tran Manh, Ngoc Tran Minh, Youn Ui Joung, Min Byung Sun, Na Minkyun, Thuong Phuong Thien, Bae Kihwan
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Biol Pharm Bull. 2008 Jan;31(1):159-62. doi: 10.1248/bpb.31.159.
Pseudocoptisine, a quaternary alkaloid with benzylisoquinoline skeleton, was isolated from Corydalis Tuber, one of the important medicinal plants in traditional medicine. Pseudocoptisine inhibited acetylcholinesterase (AChE) activity in a dose-dependent manner, and the concentration required for 50% inhibition was 12.8 microM. In further study, the anti-amnesic activities of pseudocoptisine in mice on the learning and memory impairments induced by scopolamine (1.0 mg/kg, i.p.) were examined. This alkaloid (2.0 mg/kg, p.o.) administration significantly reversed cognitive impairments in mice by passive avoidance test (p<0.05). It also reduced escape latencies in training trials and prolonged swimming times in the target quadrant during the probe trial in the water maze task (p<0.05). These results indicate that pseudocoptisine has anti-cholinesterase and anti-amnesic activities that may be useful for cognitive impairment treatment.
伪黄连碱是一种具有苄基异喹啉骨架的季铵生物碱，从延胡索（传统医学中的重要药用植物之一）中分离得到。伪黄连碱以剂量依赖的方式抑制乙酰胆碱酯酶（AChE）活性，50%抑制所需浓度为12.8微摩尔。在进一步研究中，检测了伪黄连碱对东莨菪碱（1.0毫克/千克，腹腔注射）诱导的小鼠学习和记忆损伤的抗遗忘活性。该生物碱（2.0毫克/千克，口服）给药通过被动回避试验显著逆转了小鼠的认知损伤（p<0.05）。在水迷宫任务的探索试验中，它还缩短了训练试验中的逃避潜伏期，并延长了目标象限的游泳时间（p<0.05）。这些结果表明，伪黄连碱具有抗胆碱酯酶和抗遗忘活性，可能对认知损伤治疗有用。
