El-Ries Mohamed Abdel Nabi, Mohamed Gehad Genidy, Attia Ali Kamal
National Organization for Drug Control and Research, Cairo, Egypt.
Yakugaku Zasshi. 2008 Jan;128(1):171-7. doi: 10.1248/yakushi.128.171.
The electrochemical oxidation of repaglinide has been carried out in Britton-Robinson buffer at carbon paste and glassy carbon electrodes. Repaglinide exhibits a well-defined irreversible oxidation peak over the entire pH range (2-11). Differential pulse voltammetry was used to determine repaglinide in pure form. The peak current varied linearly in the following ranges: 8.0 x 10(-7)-3.2 x 10(-6) M and 4.0 x 10(-7)-4.0 x 10(-6) M in case of carbon paste electrode and glassy carbon electrode, respectively. In case of carbon paste electrode the limits of detection (LOD) and quantification (LOQ) were 1.348 x 10(-7) M and 4.494 x 10(-7) M, respectively. For glassy carbon electrode the LOD and LOQ were 1.062 x 10(-7) M and 3.54 x 10(-7) M, respectively. The percentage recoveries were found in the following ranges: 99.09-100.07% and 99.0-100.50% for carbon paste electrode and glassy carbon electrode, respectively. The relative standard deviations were found in the following ranges: 0.636-1.395% and 0.431-1.104% in case of carbon paste electrode and glassy carbon electrode, respectively. Differential pulse voltammetry was successfully applied for the determination of repaglinide in tablets and human serum.
瑞格列奈在 Britton-Robinson 缓冲液中于碳糊电极和玻碳电极上进行了电化学氧化。瑞格列奈在整个 pH 范围（2 - 11）内呈现出明确的不可逆氧化峰。采用差分脉冲伏安法测定纯形式的瑞格列奈。在碳糊电极和玻碳电极的情况下，峰电流分别在以下范围内呈线性变化：8.0×10⁻⁷ - 3.2×10⁻⁶ M 和 4.0×10⁻⁷ - 4.0×10⁻⁶ M。对于碳糊电极，检测限（LOD）和定量限（LOQ）分别为 1.348×10⁻⁷ M 和 4.494×10⁻⁷ M。对于玻碳电极，LOD 和 LOQ 分别为 1.062×10⁻⁷ M 和 3.54×10⁻⁷ M。回收率分别在以下范围内：碳糊电极 99.09 - 100.07%，玻碳电极 99.0 - 100.50%。相对标准偏差分别在以下范围内：碳糊电极 0.636 - 1.395%，玻碳电极 0.431 - 1.104%。差分脉冲伏安法成功应用于片剂和人血清中瑞格列奈的测定。
