Mohiuddin M, Arbain D, Islam A K M Shafiqul, Ahmad M S, Ahmad M N
Production Department, Palash Urea Fertilizer Factory Ltd., Bangladesh Chemical Industries Corporation, Dhaka, Bangladesh.
School of Bioprocess Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia.
Nanoscale Res Lett. 2016 Dec;11(1):95. doi: 10.1186/s11671-016-1292-1. Epub 2016 Feb 18.
A biosensor for measuring the antidiabetic potential of medicinal plants was developed by covalent immobilization of α-glucosidase (AG) enzyme onto amine-functionalized multi-walled carbon nanotubes (MWCNTs-NH2). The immobilized enzyme was entrapped in freeze-thawed polyvinyl alcohol (PVA) together with p-nitrophenyl-α-D-glucopyranoside (PNPG) on the screen-printed carbon electrode at low pH to prevent the premature reaction between PNPG and AG enzyme. The enzymatic reaction within the biosensor is inhibited by bioactive compounds in the medicinal plant extracts. The capability of medicinal plants to inhibit the AG enzyme on the electrode correlates to the potential of the medicinal plants to inhibit the production of glucose from the carbohydrate in the human body. Thus, the inhibition indicates the antidiabetic potential of the medicinal plants. The performance of the biosensor was evaluated to measure the antidiabetic potential of three medicinal plants such as Tebengau (Ehretis laevis), Cemumar (Micromelum pubescens), and Kedondong (Spondias dulcis) and acarbose (commercial antidiabetic drug) via cyclic voltammetry, amperometry, and spectrophotometry. The cyclic voltammetry (CV) response for the inhibition of the AG enzyme activity by Tebengau plant extracts showed a linear relation in the range from 0.423-8.29 μA, and the inhibition detection limit was 0.253 μA. The biosensor exhibited good sensitivity (0.422 μA/mg Tebengau plant extracts) and rapid response (22 s). The biosensor retains approximately 82.16 % of its initial activity even after 30 days of storage at 4 °C.
通过将α-葡萄糖苷酶（AG）共价固定在胺功能化多壁碳纳米管（MWCNTs-NH2）上，开发了一种用于测量药用植物抗糖尿病潜力的生物传感器。在低pH值下，将固定化酶与对硝基苯基-α-D-吡喃葡萄糖苷（PNPG）一起包裹在冻融的聚乙烯醇（PVA）中，并置于丝网印刷碳电极上，以防止PNPG与AG酶过早反应。药用植物提取物中的生物活性化合物会抑制生物传感器内的酶促反应。药用植物抑制电极上AG酶的能力与药用植物抑制人体碳水化合物产生葡萄糖的潜力相关。因此，这种抑制作用表明了药用植物的抗糖尿病潜力。通过循环伏安法、安培法和分光光度法，对三种药用植物（如Tebengau（Ehretis laevis）、Cemumar（Micromelum pubescens）和Kedondong（Spondias dulcis））以及阿卡波糖（商业抗糖尿病药物）的抗糖尿病潜力进行了生物传感器性能评估。Tebengau植物提取物对AG酶活性抑制的循环伏安法（CV）响应在0.423 - 8.29 μA范围内呈线性关系，抑制检测限为0.253 μA。该生物传感器表现出良好的灵敏度（0.422 μA/mg Tebengau植物提取物）和快速响应（22秒）。即使在4°C下储存30天后，该生物传感器仍保留其初始活性的约82.16%。
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