Maruyama Takuro, Kamakura Hiroyuki, Kikura-Hanajiri Ruri, Goda Yukihiro
National Institute of Health Sciences, Division of Pharmacognosy, Phytochemistry and Narcotics, Kamiyoga, Setagaya-ku, Tokyo, Japan.
Yakugaku Zasshi. 2008 Jan;128(1):179-83. doi: 10.1248/yakushi.128.179.
Ultra performance liquid chromatography (UPLC)/mass spectrometry (MS) analysis was performed to investigate whether commercial Salvia cultivars available in the Japanese market contain salvinorin A (1), which is an hallucinogen present in magic mint (Salvia divinorum) prior to the regulation of S. divinorum by the Japanese Pharmaceutical Affairs Law. In addition, a previously reported method to authenticate S. divinorum, utilizing an amplification refractory mutation system (ARMS) was applied to the same samples to estimate the method's accuracy. As a result of the UPLC/MS analysis, it was clear that none of the tested cultivars possessed 1 while S. divinorum leaves and its processed products "concentrated salvia" contained 1 in the range from 0.19% to 0.58%. Furthermore, the ARMS method could clearly distinguish S. divinorum from the tested cultivars. In conclusion, the authentication method is considered to be useful for the practical regulation of S. divinorum due to its simplicity and accuracy.
采用超高效液相色谱（UPLC）/质谱（MS）分析法，调查日本市场上销售的丹参栽培品种是否含有迷幻剂丹参酮A（1），该物质存在于魔法薄荷（Salvia divinorum）中，在日本《药事法》对Salvia divinorum进行管制之前。此外，将先前报道的利用扩增阻滞突变系统（ARMS）鉴定Salvia divinorum的方法应用于相同样品，以评估该方法的准确性。UPLC/MS分析结果表明，所测试的栽培品种均不含有1，而Salvia divinorum叶片及其加工产品“浓缩丹参”中1的含量在0.19%至0.58%之间。此外，ARMS方法能够清晰地区分Salvia divinorum与所测试的栽培品种。总之，该鉴定方法因其简单性和准确性，被认为对Salvia divinorum的实际管制有用。
