Pichini Simona, Abanades Sergio, Farré Magí, Pellegrini Manuela, Marchei Emilia, Pacifici Roberta, Torre Rafael de la, Zuccaro Piergiorgio
Drug Research and Evaluation Department, Istituto Superiore di Sanità. Rome, Italy.
Rapid Commun Mass Spectrom. 2005;19(12):1649-56. doi: 10.1002/rcm.1970.
A gas chromatography method with mass spectrometric detection is described for the determination of Salvinorin A, the main active ingredient of the hallucinogenic mint Salvia divinorum. The method was validated in plasma, urine, saliva and sweat using 17-alpha-methyltestosterone as internal standard. The analytes were extracted from biological matrices with chloroform/isopropanol (9:1, v/v). Chromatography was performed on a 5% phenyl methyl silicone capillary column and analytes were determined in the selected ion monitoring mode. The method was validated over the concentration range 0.015-5 microg/mL plasma, urine and saliva and 0.01-5 microg/patch in the case of sweat. Mean recoveries ranged between 77.1 and 92.7% for Salvinorin A in different biological matrices, with precision and accuracy always better than 15%. The method was applied to the analysis of urine, saliva and sweat from two consumers after smoking 75 mg plant leaves to verify the presence of the active ingredient of S. divinorum in human biological fluids as a biomarker of plant consumption. Salvinorin A was detected in urine (2.4 and 10.9 ng/mL) and saliva (11.1 and 25.0 ng/mL), but not in sweat patches from consumers.
描述了一种采用质谱检测的气相色谱法，用于测定致幻薄荷鼠尾草（Salvia divinorum）的主要活性成分Salvinorin A。该方法以17-α-甲基睾酮作为内标，在血浆、尿液、唾液和汗液中进行了验证。采用氯仿/异丙醇（9:1，v/v）从生物基质中提取分析物。在5%苯基甲基硅氧烷毛细管柱上进行色谱分析，并在选择离子监测模式下测定分析物。该方法在血浆、尿液和唾液的浓度范围为0.015 - 5 μg/mL，汗液的浓度范围为0.01 - 5 μg/贴片的条件下进行了验证。Salvinorin A在不同生物基质中的平均回收率在77.1%至92.7%之间，精密度和准确度始终优于15%。该方法应用于两名吸食75 mg植物叶片的消费者的尿液、唾液和汗液分析，以验证鼠尾草活性成分在人体生物体液中的存在，作为植物消费的生物标志物。在尿液（2.4和10.9 ng/mL）和唾液（11.1和25.0 ng/mL）中检测到了Salvinorin A，但在消费者的汗液贴片中未检测到。
