无标记阻抗生物传感器：机遇与挑战
Label-Free Impedance Biosensors: Opportunities and Challenges.
作者信息
Daniels Jonathan S, Pourmand Nader
机构信息
Stanford Genome Technology Center; 855 S. California Ave., Palo Alto, CA 94304, USA.
出版信息
Electroanalysis. 2007 May 16;19(12):1239-1257. doi: 10.1002/elan.200603855.
Abstract
Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.
摘要
阻抗生物传感器是一类电化学生物传感器，因其成本低、易于小型化和无需标记操作，在即时检测及其他应用中显示出前景。当靶分子与固定化探针结合时，通过监测表面阻抗的变化可检测未标记的DNA和蛋白质靶标。亲和捕获步骤带来了所有无标记亲和生物传感器共有的挑战；这些挑战将与阻抗读出特有的其他挑战一起讨论。还讨论了靶标结合后阻抗变化的各种可能机制。我们批判性地总结了过去无标记阻抗生物传感器的成果，并确定了未来的研究领域。
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