RNA位点特异性修饰的联合方法
Combined Approaches to Site-Specific Modification of RNA.
作者信息
Chow Christine S, Mahto Santosh K, Lamichhane Tek N
出版信息
ACS Chem Biol. 2008 Jan 18;3(1):30-37. doi: 10.1021/cb7002225. Epub 2008 Jan 5.
Abstract
Both natural and unnatural modifications in RNA are of interest to biologists and chemists. More than 100 different analogues of the four standard RNA nucleosides have been identified in nature. Unnatural modifications are useful for structure and mechanistic studies of RNA. This Review highlights chemical, enzymatic, and combined (semisynthesis) approaches to generate site specifically modified RNAs. The availability of these methods for site-specific modifications of RNAs of all sizes is important in order to study the relationships between RNA chemical composition, structure, and function.
摘要
RNA中的天然修饰和非天然修饰都引起了生物学家和化学家的兴趣。自然界中已鉴定出100多种四种标准RNA核苷的不同类似物。非天然修饰对于RNA的结构和机制研究很有用。本综述重点介绍了生成位点特异性修饰RNA的化学、酶促和联合（半合成）方法。为了研究RNA化学组成、结构和功能之间的关系，所有大小的RNA位点特异性修饰方法的可用性都很重要。
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