Lu Pei-Lin, Lai Jui-Yang, Ma David Hui-Kang, Hsiue Ging-Ho
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013, Republic of China.
J Biomater Sci Polym Ed. 2008;19(1):1-18. doi: 10.1163/156856208783227695.
It was reported that cell-adhesive gelatin discs have been successfully used as delivery vehicles for intraocular grafting of bioengineered corneal endothelial cell sheets. Development of alternative biomaterials to bovine-based gelatin vehicles can potentially eliminate the risk of bovine spongiform encephalopathy. In the present work, to investigate whether it was appropriate for use as cell sheet delivery vehicles, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) cross-linked hyaluronic acid (HA) hydrogels were studied by determinations of morphological characteristic, mechanical and thermal property, water content, in vitro degradability and cytocompatibility. Glutaraldehyde (GTA) cross-linked HA samples were used for comparison. It was found that HA discs after cross-linking significantly increased its tensile stress but reduced its tensile strain, water uptake and enzymatic degradability. The results of differential scanning calorimetry demonstrated that cross-linking could lead to the alteration of polymer structure. In addition, the EDC-cross-linked HA discs had a smoother surface structure, a faster degradation rate and a relatively lower cytotoxicity as compared to the GTA cross-linked counterparts. It is concluded that EDC can be successfully applied for HA cross-linking to fabricate structurally stable, mechanically reinforced, readily deformable, transparent and cytocompatible HA hydrogel discs with the potential to be applied as delivery vehicles for corneal endothelial cell therapy.
据报道，细胞粘附性明胶圆盘已成功用作生物工程角膜内皮细胞片眼内移植的递送载体。开发替代牛源明胶载体的生物材料可能会消除牛海绵状脑病的风险。在本研究中，为了研究其是否适合用作细胞片递送载体，通过测定形态特征、力学和热性能、含水量、体外降解性和细胞相容性，对1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)交联的透明质酸(HA)水凝胶进行了研究。使用戊二醛(GTA)交联的HA样品作为对照。结果发现，交联后的HA圆盘显著增加了其拉伸应力，但降低了其拉伸应变、吸水率和酶降解性。差示扫描量热法的结果表明，交联会导致聚合物结构的改变。此外，与GTA交联的HA圆盘相比，EDC交联的HA圆盘具有更光滑的表面结构、更快的降解速率和相对较低的细胞毒性。结论是，EDC可成功应用于HA交联，以制备结构稳定、机械增强、易于变形、透明且细胞相容的HA水凝胶圆盘，有潜力用作角膜内皮细胞治疗的递送载体。
