Department of Mechanical Engineering, Chosun University, GwangJu, South Korea.
Biomacromolecules. 2012 Sep 10;13(9):2997-3003. doi: 10.1021/bm3011352. Epub 2012 Aug 30.
In this study, we propose a new cell encapsulation method consisting of a dispensing method and an aerosol-spraying method. The aerosol spray using a cross-linking agent, calcium chloride (CaCl(2)), was used to control the surface gelation of dispensed alginate struts during dispensing. To show the feasibility of the method, we used preosteoblast (MC3T3-E1) cells. By changing the relationship between the various dispensing/aerosol-spraying conditions and cell viability, we could determine the optimal cell-dispensing process: a nozzle size (240 μm) and an aerosol spray flow rate (0.93 ± 0.12 mL min(-1)), 10 mm s(-1) nozzle moving speed, a 10 wt % concentration of CaCl(2) in the aerosol solution, and 2 wt % concentration of CaCl(2) in the second cross-linking process. Based on these optimized process conditions, we successfully fabricated a three-dimensional, pore-structured, cell-laden alginate scaffold of 20 × 20 × 4.6 mm(3) and 84% cell viability. During long cell culture periods (16, 25, 33, and 45 days), the preosteoblasts in the alginate scaffold survived and proliferated well.
在这项研究中，我们提出了一种新的细胞包封方法，包括分配方法和喷雾方法。使用交联剂氯化钙 (CaCl(2)) 的喷雾用于控制分配过程中藻酸盐支柱的表面胶凝。为了展示该方法的可行性，我们使用了前成骨细胞 (MC3T3-E1) 细胞。通过改变各种分配/喷雾条件与细胞活力之间的关系，我们可以确定最佳的细胞分配过程：喷嘴尺寸（240 μm）和喷雾流速（0.93 ± 0.12 mL min(-1))，喷嘴移动速度为 10 mm s(-1)，喷雾溶液中 CaCl(2) 的浓度为 10 wt %，第二交联过程中 CaCl(2) 的浓度为 2 wt %。基于这些优化的工艺条件，我们成功地制造了一种三维、多孔、细胞负载的藻酸盐支架，尺寸为 20×20×4.6 mm(3)，细胞活力为 84%。在长时间的细胞培养期间（16、25、33 和 45 天），藻酸盐支架中的前成骨细胞存活并良好增殖。
