Sempere F, Santamarina M P
Department of Agroforest Ecosystems, School of Rural Environments and Enology, Polytechnic University of Valencia, Avda. Blasco Ibañez, 21, 46010-Valencia, Spain.
Int J Food Microbiol. 2008 Feb 29;122(1-2):35-43. doi: 10.1016/j.ijfoodmicro.2007.11.080. Epub 2007 Dec 4.
The objective of this research was to study by means of different techniques, the interaction between Penicillium oxalicum and Nigrospora oryzae under different temperatures (15 and 25 degrees C), water activities (0.95, 0.98, and 0.995) and culture media (rice and rice extract agar). In dual culture, P. oxalicum was dominant over N. oryzae in spite of presenting in the majority of cases, lower growth rates. The microscopic study revealed that P. oxalicum is a powerful mycoparasite, which attacks the conidiophores and the spores of N. oryzae, not only surrounding them, but also penetrating, deforming, destroying and developing reproductive structures inside them. The antagonist did not change its way of performance in the different tested conditions. Water activity and temperature showed a significant effect on fungus growth.
本研究的目的是通过不同技术，研究在不同温度（15和25摄氏度）、水分活度（0.95、0.98和0.995）及培养基（大米和大米提取物琼脂）条件下，草酸青霉与稻黑孢之间的相互作用。在共培养中，尽管在大多数情况下草酸青霉的生长速率较低，但它在与稻黑孢的竞争中占主导地位。显微镜研究表明，草酸青霉是一种强大的真菌寄生物，它攻击稻黑孢的分生孢子梗和孢子，不仅包围它们，还穿透、变形、破坏并在其中发育繁殖结构。在不同的测试条件下，拮抗菌的作用方式没有改变。水分活度和温度对真菌生长有显著影响。
