Naik B Shankar, Shashikala J, Krishnamurthy Y L
Department of PG Studies and Research in Applied Botany, Bio-Science Complex, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shimoga District, Karnataka, India.
Microbiol Res. 2009;164(3):290-6. doi: 10.1016/j.micres.2006.12.003. Epub 2007 Apr 12.
Endophytic populations were isolated from 2400 segments of Oryza sativa collected from Bhadra River Project Area, Southern India during December 2005 (Winter) and April 2006 (Summer). Overall colonization rates from surface sterilized tissues were 40.3% in roots and 25.83% in leaves during winter season, 20.15% in roots and 8.66% in leaves during summer season. Nineteen different fungal taxa, a Streptomyces sp. and bacterial species were isolated. Streptomyces sp., Chaetomium globosum, Penicillium chrysogenum, Fusarium oxysporum and Cladosporium cladosporioides were dominant endophytes in this study. Frequency of colonization between the sites, seasons and rice varieties were found to differ significantly. Dual culture studies revealed that C. globosum, P. chrysogenum and Streptomyces sp. are suitable candidates for extraction of biologically active compounds. Rice harbors many endophytic organisms and some of them have antagonistic properties against fungal pathogens.
2005年12月（冬季）和2006年4月（夏季），从印度南部巴德拉河项目区采集的2400段水稻中分离出内生菌群。冬季，经表面消毒处理的组织中，根部的总体定殖率为40.3%，叶片为25.83%；夏季，根部为20.15%，叶片为8.66%。分离出了19种不同的真菌分类群、1株链霉菌属菌株和细菌种类。在本研究中，链霉菌属菌株、球毛壳菌、产黄青霉、尖孢镰刀菌和枝孢枝孢菌是主要的内生菌。发现不同地点、季节和水稻品种之间的定殖频率存在显著差异。共培养研究表明，球毛壳菌、产黄青霉和链霉菌属菌株是提取生物活性化合物的合适候选菌株。水稻含有许多内生生物，其中一些对真菌病原体具有拮抗特性。
