Li Hong
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.
Curr Opin Struct Biol. 2008 Feb;18(1):78-85. doi: 10.1016/j.sbi.2007.11.004.
The H/ACA RNP pseudouridylases function on a large number of extraordinarily complex RNA substrates including pre-ribosomal and small nuclear RNAs. Recent structural data show that H/ACA RNPs capture their RNA substrates via a simple one-sided attachment model. However, the precise placement of each RNA substrate into the active site of the catalytic subunit relies on the essential functions of the RNP proteins. The specific roles of each H/ACA RNP protein are being elucidated by a combination of structural and biochemical studies.
H/ACA核糖核蛋白假尿苷酰化酶作用于大量极其复杂的RNA底物，包括前核糖体RNA和小核RNA。最近的结构数据表明，H/ACA核糖核蛋白通过一种简单的单侧附着模型捕获其RNA底物。然而，将每个RNA底物精确放置到催化亚基的活性位点依赖于核糖核蛋白的基本功能。通过结构和生化研究相结合的方式，正在阐明每个H/ACA核糖核蛋白的具体作用。
