da Silva Luciana Helena Antoniassi, Spilki Fernando Rosado, Riccetto Adriana Gut Lopes, de Almeida Renata Servan, Baracat Emílio Carlos Elias, Arns Clarice Weis
Faculdade de Ciências Médicas, Programa Pós Graduação - Clínica Médica - UNICAMP, Campinas, Brazil.
J Clin Virol. 2008 May;42(1):78-81. doi: 10.1016/j.jcv.2007.11.010. Epub 2008 Jan 7.
Human metapneumovirus (HMPV) is a paramyxovirus associated with respiratory illness. The genotypes of HMPV isolates in Brazil have not been well characterized.
To investigate the presence of HMPV in clinical samples collected from pediatric patients of two university hospitals in the region of Campinas (São Paulo, Brazil) and to genotype them by partial sequencing of the HMPV F gene.
Nasopharyngeal aspirates were collected from children hospitalized between April and September, 2004 because of acute respiratory infections (ARI).
We identified HMPV in 8 of 142 (5.6%) clinical samples. We determined through phylogenetic analysis that HMPV isolates in Campinas during the study were clustered within subgroup B genotype 1. Two of the isolates analyzed showed significant differences from previously isolated B1 viruses, when compared to HMPV isolated in South Africa and Canada, and clustered in a separate branch within this genotype.
In 2004 in our geographic region all HMPV isolates from pediatric patients were in the B1 HMPV genetic group, with two variant isolates.
人偏肺病毒（HMPV）是一种与呼吸道疾病相关的副粘病毒。巴西HMPV分离株的基因型尚未得到充分表征。
调查从坎皮纳斯地区（巴西圣保罗）两家大学医院的儿科患者收集的临床样本中HMPV的存在情况，并通过对HMPV F基因进行部分测序对其进行基因分型。
收集了2004年4月至9月期间因急性呼吸道感染（ARI）住院的儿童的鼻咽抽吸物。
我们在142份临床样本中的8份（5.6%）中鉴定出HMPV。通过系统发育分析，我们确定研究期间坎皮纳斯的HMPV分离株聚集在B亚组基因型1内。与在南非和加拿大分离的HMPV相比，分析的两个分离株与先前分离的B1病毒存在显著差异，并聚集在该基因型内的一个单独分支中。
2004年在我们的地理区域，来自儿科患者的所有HMPV分离株都属于B1 HMPV基因组，有两个变异分离株。
