Haddad Renato, Sparrapan Regina, Kotiaho Tapio, Eberlin Marcos N
ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, 13084-971, Campinas SP, Brazil.
Anal Chem. 2008 Feb 1;80(3):898-903. doi: 10.1021/ac701960q. Epub 2008 Jan 8.
Using a cellulose dialysis membrane and aqueous solutions of common drugs as a proof-of-principle example, we demonstrate that solid but permeable and flexible membranes can be used as interfaces for the direct analysis of solution constituents via easy ambient sonic-spray ionization mass spectrometry. This new combination of MS techniques, herein termed EASI-MIMS, promotes droplet pick up of the analyte from the external surface of the membrane from where the analyte has selectively permeated for proper mass spectrometry characterization and quantitation. Possible application of EASI-MIMS such as the environmental analyses of effluents, on-line monitoring of fermentation and biotransformations and on-line pharmacokinetic blood analysis are discussed.
以纤维素透析膜和常见药物的水溶液作为原理验证示例，我们证明了固态但可渗透且柔韧的膜可作为接口，通过简便的常压声波喷雾电离质谱法直接分析溶液成分。这种新的质谱技术组合，在此称为EASI-MIMS，可促进从膜外表面拾取分析物液滴，分析物已从该表面选择性渗透，以便进行适当的质谱表征和定量。文中还讨论了EASI-MIMS的可能应用，如废水的环境分析、发酵和生物转化的在线监测以及药代动力学血液在线分析。
