Haddad Renato, Milagre Humberto M S, Catharino Rodrigo Ramos, Eberlin Marcos N
ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, 13084-971, Campinas SP, Brazil.
Anal Chem. 2008 Apr 15;80(8):2744-50. doi: 10.1021/ac702216q. Epub 2008 Mar 11.
On-spot detection and analyte characterization on thin-layer chromatography (TLC) plates is performed via ambient desorption/ionization and (tandem) mass spectrometry detection, that is, via easy ambient sonic spray ionization mass spectrometry (EASI-MS). As proof-of-principle cases, mixtures of semipolar nitrogenated compounds as well as pharmaceutical drugs and vegetable oils have been tested. The technique has also been applied to monitor a chemical reaction of synthetic importance. EASI is the simplest and gentlest ambient ionization technique currently available, assisted solely by N2 (or air). It uses no voltages, no electrical discharges; no UV or laser beams, and no high temperature and is most easily implemented in all API mass spectrometers. TLC is also the simplest, fastest, and most easily performed chromatographic technique. TLC plus EASI-MS therefore provide a simple and advantageous combination of chromatographic separation and sensitive detection of the TLC spots as well as on-spot MS or MS/MS characterization. The favorable characteristics of TLC-EASI-MS indicate advantageous applications in several areas such as drug and oil analysis, phytochemistry and synthetic chemistry, forensics via reliable counterfeit detection, and quality control.
通过常压解吸/电离和（串联）质谱检测，即在常压超声喷雾电离质谱（EASI-MS）的辅助下，可在薄层色谱（TLC）板上进行现场检测和分析物表征。作为原理验证案例，已对半极性含氮化合物混合物以及药物和植物油进行了测试。该技术还被应用于监测具有合成重要性的化学反应。EASI是目前可用的最简单、最温和的常压电离技术，仅由氮气（或空气）辅助。它不使用电压、不产生放电；不使用紫外线或激光束，也不涉及高温，并且最容易在所有API质谱仪中实现。TLC也是最简单、最快且最易于操作的色谱技术。因此，TLC与EASI-MS相结合，为色谱分离以及TLC斑点的灵敏检测和现场MS或MS/MS表征提供了一种简单且有利的组合。TLC-EASI-MS的良好特性表明其在药物和油类分析、植物化学和合成化学、通过可靠的假冒检测进行法医学以及质量控制等多个领域具有优势应用。
