Fats and Oils Laboratory, Faculty of Food Engineering, University of Campinas - UNICAMP, Campinas 13083-970, SP, Brazil.
Food Chem. 2012 Dec 1;135(3):1855-60. doi: 10.1016/j.foodchem.2012.06.072. Epub 2012 Jun 29.
Commercial lecithins are composed mainly of phospholipids and triacylglycerols. The analysis of the commercial lecithins, including their fraction of phospholipids, normally involves laborious and expensive protocols. Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is shown to be an efficient technique for the analysis of lipids. Samples of commercial lecithins including standards, refined, deoiled and modified soy lecithin were tested. Characteristic profiles of phosphatidylcholines and triacylglycerols are detected by EASI(+)-MS, whereas EASI(-)-MS provided phosphatidylethanolamines, glycophospholipids and free fatty acids profiles. Acetylated lecithins also displayed characteristic acetylated derivatives. EASI-MS data was also compared to MALDI-MS, and found to display richer compositional information. The industrial process applied to lecithin fabrication was also characterised via typical EASI-MS profiles. EASI-MS both in its positive and negative ion modes offers a direct, fast and efficient technique able to characterise commercial lecithin.
商业级卵磷脂主要由磷脂和三酰基甘油组成。商业级卵磷脂的分析，包括其磷脂的比例，通常需要繁琐和昂贵的方案。简单环境超声喷雾电离质谱（EASI-MS）被证明是一种分析脂质的有效技术。对包括标准品、精炼品、脱油和改性大豆卵磷脂在内的商业级卵磷脂样品进行了测试。EASI(+)-MS 检测到了磷脂酰胆碱和三酰基甘油的特征图谱，而 EASI(-)-MS 则提供了磷脂酰乙醇胺、糖脂和游离脂肪酸的图谱。乙酰化卵磷脂也显示出特征性的乙酰化衍生物。EASI-MS 数据也与 MALDI-MS 进行了比较，发现其具有更丰富的组成信息。还通过典型的 EASI-MS 图谱对卵磷脂制造的工业过程进行了表征。EASI-MS 无论是正离子模式还是负离子模式，都提供了一种直接、快速和有效的技术，能够对商业级卵磷脂进行表征。
