Danker Tanja, Dreesen Björn, Offermann Sascha, Horst Ina, Peterhänsel Christoph
Rheinisch-Westfälische Hochschule Aachen, Biology I, 52056 Aachen, Germany.
Plant J. 2008 Feb;53(3):465-74. doi: 10.1111/j.1365-313X.2007.03352.x. Epub 2008 Jan 4.
We have investigated the establishment of histone H3 methylation with respect to environmental and developmental signals for two key genes associated with C4 photosynthesis in maize. Tri-methylation of histone H3 lysine 4 (H3K4) in roots and leaves was shown to be controlled by autonomous cell-type-specific developmental signals that are independent of illumination and therefore independent of the initiation of transcription. Di- and mono-methylation of H3K4 act antagonistically to this process. The modifications were already established in etiolated seedlings, and remained stable when genes were inactivated by dark treatment or pharmaceutical inhibition of transcription. Constitutive di-methylation of H3K9 was concomitantly detected at specific gene positions. The data support a histone code model whereby cell-type-specific signals induce the formation of a chromatin structure that potentiates gene activation by environmental cues.
我们针对玉米中与C4光合作用相关的两个关键基因，研究了组蛋白H3甲基化与环境和发育信号的关系。结果表明，根和叶中组蛋白H3赖氨酸4（H3K4）的三甲基化受自主细胞类型特异性发育信号控制，这些信号独立于光照，因此也独立于转录起始。H3K4的二甲基化和单甲基化对此过程起拮抗作用。这些修饰在黄化幼苗中已经建立，并且当基因通过黑暗处理或药物抑制转录而失活时仍保持稳定。同时在特定基因位置检测到H3K9的组成型二甲基化。这些数据支持一种组蛋白编码模型，即细胞类型特异性信号诱导染色质结构的形成，该结构通过环境线索增强基因激活。
