Valledor Luis, Pascual Jesús, Meijón Mónica, Escandón Mónica, Cañal María Jesús
Plant Physiology, Faculty of Biology, University of Oviedo, Cat. Rodrígo Uría s/n, E-33071, Oviedo, Spain; Department of Biology and CESAM, University of Aveiro, Campus Universitario de Santiago, P-3810-193, Aveiro, Portugal.
Plant Physiology, Faculty of Biology, University of Oviedo, Cat. Rodrígo Uría s/n, E-33071, Oviedo, Spain.
PLoS One. 2015 May 12;10(5):e0126405. doi: 10.1371/journal.pone.0126405. eCollection 2015.
Needle maturation is a complex process that involves cell growth, differentiation and tissue remodelling towards the acquisition of full physiological competence. Leaf induction mechanisms are well known; however, those underlying the acquisition of physiological competence are still poorly understood, especially in conifers. We studied the specific epigenetic regulation of genes defining organ function (PrRBCS and PrRBCA) and competence and stress response (PrCSDP2 and PrSHMT4) during three stages of needle development and one de-differentiated control. Gene-specific changes in DNA methylation and histone were analysed by bisulfite sequencing and chromatin immunoprecipitation (ChIP). The expression of PrRBCA and PrRBCS increased during needle maturation and was associated with the progressive loss of H3K9me3, H3K27me3 and the increase in AcH4. The maturation-related silencing of PrSHMT4 was correlated with increased H3K9me3 levels, and the repression of PrCSDP2, to the interplay between AcH4, H3K27me3, H3K9me3 and specific DNA methylation. The employ of HAT and HDAC inhibitors led to a further determination of the role of histone acetylation in the regulation of our target genes. The integration of these results with high-throughput analyses in Arabidopsis thaliana and Populus trichocarpa suggests that the specific epigenetic mechanisms that regulate photosynthetic genes are conserved between the analysed species.
针叶成熟是一个复杂的过程，涉及细胞生长、分化以及组织重塑，以获得完全的生理能力。叶片诱导机制已为人熟知；然而，关于获得生理能力的潜在机制仍知之甚少，尤其是在针叶树中。我们研究了在针叶发育的三个阶段以及一个去分化对照过程中，定义器官功能（PrRBCS和PrRBCA）、能力和应激反应（PrCSDP2和PrSHMT4）的基因的特定表观遗传调控。通过亚硫酸氢盐测序和染色质免疫沉淀（ChIP）分析了DNA甲基化和组蛋白的基因特异性变化。PrRBCA和PrRBCS的表达在针叶成熟过程中增加，并且与H3K9me3、H3K27me3的逐渐丧失以及AcH4的增加相关。PrSHMT4的成熟相关沉默与H3K9me3水平的增加相关，而PrCSDP2的抑制则与AcH4、H3K27me3、H3K9me3和特定DNA甲基化之间的相互作用有关。使用组蛋白乙酰转移酶（HAT）和组蛋白去乙酰化酶（HDAC）抑制剂进一步确定了组蛋白乙酰化在调控我们的靶基因中的作用。将这些结果与拟南芥和毛果杨的高通量分析相结合，表明调控光合基因的特定表观遗传机制在所分析的物种之间是保守的。
