Mangell Peter, Lennernäs Pernilla, Wang Mei, Olsson Crister, Ahrné Siv, Molin Göran, Thorlacius Henrik, Jeppsson Bengt
Department of Surgery, Malmö University Hospital, Lund University, Malmö, Sweden.
APMIS. 2006 Sep;114(9):611-8. doi: 10.1111/j.1600-0463.2006.apm_369.x.
The preventive effect of the probiotic Lactobacillus plantarum 299v on bacterial translocation (BT) and the role of adhesion were studied in septic rats. Five groups of rats were pretreated as follows: negative and positive control groups received regular drinking water; the oatmeal group received drinking water mixed with oatmeal; the Lp 299v group received drinking water mixed with oatmeal containing 10(9) colony-forming units (CFU) L. plantarum 299v/ml; the Lp 299v-adh(-) group received drinking water with oatmeal containing 10(9) CFU/ml of modified L. plantarum 299v (L. plantarum 299v-adh(-)) lacking adhesive properties to enterocytes. On day 8, all rats except the negative control group were given lipopolysaccharide (LPS) intraperitoneally. After 24 h, mesenteric lymph node (MLN), liver and ileum were harvested for culture. Incidence of BT after LPS challenge was 25% and 88% in MLN and liver, respectively. BT increased to 75% in MLN and 100% in liver of endotoxemic rats pretreated with oatmeal. Pretreatment with L. plantarum 299v reduced BT to 0% and 12% in MLN and liver, respectively. L. plantarum 299v-adh(-) did not prevent BT to MLN. Flow cytometry revealed reduced adherence of these bacteria to intestinal epithelial cells compared to L. plantarum 299v. Thus, L. plantarum 299v prevents BT in septic rats, an effect probably dependent on bacterial adherence to the intestinal mucosa. Further, our findings indicate that oatmeal (prebiotics) without probiotics does not prevent BT during sepsis.
研究了益生菌植物乳杆菌299v对脓毒症大鼠细菌移位（BT）的预防作用及黏附的作用。将五组大鼠进行如下预处理：阴性和阳性对照组给予常规饮用水；燕麦片组给予与燕麦片混合的饮用水；植物乳杆菌299v组给予与含有10⁹菌落形成单位（CFU）/ml植物乳杆菌299v的燕麦片混合的饮用水；植物乳杆菌299v-adh(-)组给予与含有10⁹CFU/ml缺乏对肠上皮细胞黏附特性的改良植物乳杆菌299v（植物乳杆菌299v-adh(-)）的燕麦片混合的饮用水。在第8天，除阴性对照组外的所有大鼠腹腔注射脂多糖（LPS）。24小时后，采集肠系膜淋巴结（MLN）、肝脏和回肠进行培养。LPS攻击后，MLN和肝脏中BT的发生率分别为25%和88%。用燕麦片预处理的内毒素血症大鼠的MLN中BT增加到75%，肝脏中增加到100%。用植物乳杆菌299v预处理可使MLN和肝脏中的BT分别降至0%和12%。植物乳杆菌299v-adh(-)不能预防细菌向MLN的移位。流式细胞术显示，与植物乳杆菌299v相比，这些细菌对肠上皮细胞的黏附减少。因此，植物乳杆菌299v可预防脓毒症大鼠的细菌移位，这一作用可能取决于细菌对肠黏膜的黏附。此外，我们的研究结果表明，不含益生菌的燕麦片（益生元）在脓毒症期间不能预防细菌移位。
