Fujian Maternity and Child Health Hospital Affiliated to Fujian Medical University, Fuzhou, 350000, P. R. China.
Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, P. R. China.
J Microbiol. 2021 Sep;59(9):827-839. doi: 10.1007/s12275-021-1094-8. Epub 2021 Aug 12.
Probiotics effectively prevent and improve metabolic diseases such as diabetes by regulating the intestinal microenvironment and gut microbiota. However, the effects of probiotics in gestational diabetes mellitus are not clear. Here, we showed that probiotic supplements significantly improved fasting blood glucose in a gestational diabetes mellitus rat model. To further understand the mechanisms of probiotics in gestational diabetes mellitus, the gut microbiota were analyzed via 16S rRNA sequencing. We found that compared with the normal pregnant group, the gestational diabetes mellitus rats had decreased diversity of gut microbiota. Moreover, probiotic supplementation restored the diversity of the gut microbiota in gestational diabetes mellitus rats, and the gut microbiota structure tended to be similar to that of normal pregnant rats. In particular, compared with gestational diabetes mellitus rats, the abundance of Firmicutes and Actinobacteria was higher after probiotic supplementation. Furthermore, activating carbohydrate metabolism and membrane transport pathways may be involved in the potential mechanisms by which probiotic supplements alleviate gestational diabetes mellitus. Overall, our results suggested that probiotic supplementation might be a novel approach to restore the gut microbiota of gestational diabetes mellitus rats and provided an experimental evidence for the use of probiotic supplements to treat gestational diabetes mellitus.
益生菌通过调节肠道微环境和肠道微生物群，有效地预防和改善糖尿病等代谢疾病。然而，益生菌在妊娠糖尿病中的作用尚不清楚。在这里，我们表明益生菌补充剂可显著改善妊娠糖尿病大鼠模型的空腹血糖。为了进一步了解益生菌在妊娠糖尿病中的作用机制，我们通过 16S rRNA 测序分析了肠道微生物群。我们发现，与正常妊娠组相比，妊娠糖尿病大鼠的肠道微生物群多样性降低。此外，益生菌补充剂恢复了妊娠糖尿病大鼠肠道微生物群的多样性，并且肠道微生物群结构趋于与正常妊娠大鼠相似。特别是，与妊娠糖尿病大鼠相比，益生菌补充后厚壁菌门和放线菌门的丰度更高。此外，激活碳水化合物代谢和膜转运途径可能参与了益生菌补充缓解妊娠糖尿病的潜在机制。总的来说，我们的结果表明，益生菌补充可能是一种恢复妊娠糖尿病大鼠肠道微生物群的新方法，并为使用益生菌补充剂治疗妊娠糖尿病提供了实验证据。
