Inoue K, Takano H, Yanagisawa R, Sakurai M, Abe S, Yoshino S, Yamaki K, Yoshikawa T
Environmental Health Sciences Division, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan.
Int J Immunopathol Pharmacol. 2007 Oct-Dec;20(4):737-44. doi: 10.1177/039463200702000409.
Ambient particulate matter (PM) exacerbates allergic airway diseases. Our previous study showed that diesel exhaust particles, the main constituents in urban PM, enhance airway hyperresponsivness in mice. In addition, health effects of PM with a diameter of less than 100 nm, called nanoparticles, have been reported, and we have also demonstrated that carbon nanoparticles exacerbate antigen-related airway inflammation. The present study investigates the effects of pulmonary exposure to two sizes of carbon nanoparticles on lung physiology and lung expression of Muc5ac in the presence or absence of antigen in mice. Nanoparticles alone or ovalbumin (OVA) alone moderately enhanced cholinergic airway reactivity, as assessed by total respiratory system resistance (R) and Newtonian resistance (Rn). In the nanoparticle + OVA groups, all the parameters for lung responsiveness, such as R, compliance, elastance, Rn, tissue damping, and tissue elastance, were worse than those in the vehicle group, the corresponding nanoparticle groups or the OVA group. The lung mRNA level for Muc5ac was significantly higher in the OVA group than in the vehicle group, and further increased in the nanoparticle + OVA groups than in the OVA or the nanoparticle groups. These data suggest that carbon nanoparticles can enhance lung hyperresponsiveness, especially in the presence of antigen. The effects may be mediated, at least partly, through the enhanced lung expression of Muc5ac.
环境颗粒物（PM）会加剧过敏性气道疾病。我们之前的研究表明，城市PM的主要成分柴油尾气颗粒会增强小鼠的气道高反应性。此外，已有报道称直径小于100nm的颗粒物（称为纳米颗粒）对健康有影响，并且我们也已证明碳纳米颗粒会加剧抗原相关的气道炎症。本研究调查了在有或没有抗原存在的情况下，肺部暴露于两种尺寸的碳纳米颗粒对小鼠肺生理学和Muc5ac肺表达的影响。单独的纳米颗粒或单独的卵清蛋白（OVA）会适度增强胆碱能气道反应性，这通过总呼吸系统阻力（R）和牛顿阻力（Rn）来评估。在纳米颗粒+OVA组中，所有肺反应性参数，如R、顺应性、弹性、Rn、组织阻尼和组织弹性，都比载体组、相应的纳米颗粒组或OVA组更差。OVA组中Muc5ac的肺mRNA水平显著高于载体组，并且在纳米颗粒+OVA组中比在OVA组或纳米颗粒组中进一步升高。这些数据表明碳纳米颗粒可增强肺高反应性，尤其是在有抗原存在的情况下。这些影响可能至少部分是通过增强Muc5ac的肺表达来介导的。
