Ozaki Keiko, Ohta Akemi, Iwata Chieko, Horikawa Aki, Tsuji Kiyomi, Ito Emiko, Ikai Yoshitomo, Harada Ken-Ichi
Graduate School of Environmental and Human Science, Meijo University, Tempaku, Nagoya 468-8503, Japan.
Chemosphere. 2008 Apr;71(8):1531-8. doi: 10.1016/j.chemosphere.2007.11.052. Epub 2008 Jan 7.
One of bacteria collected from Lake Sagami, Japan, Brevibacillus sp., was found to have a lytic activity of cyanobacteria, but did not produce active compounds. Instead, the co-culturing of Microcystis with the Brevibacillus sp. enhanced the production of two volatile compounds, beta-cyclocitral and 3-methyl-1-butanol, and the former had a characteristic lytic activity. It was confirmed that these volatile compounds were derived from the cyanobacteria themselves. beta-Ionone, geosmin and 2-methylisoborneol derived from cyanobacteria and similar volatile compounds, terpenoids, produced by plants also had a lytic activity. The minimum inhibitory concentration values of the cyanobacterial metabolites were estimated to be higher than those of compounds from plants except for a few compounds. Among them, beta-cyclocitral only produced a characteristic color change of culture broth from green to blue. This color change is similar to the phenomenon observed when a sudden decline in growth of cyanobacteria begins in a natural environment.
从日本相模湖采集的一种细菌——短短芽孢杆菌（Brevibacillus sp.），被发现具有蓝藻裂解活性，但不产生活性化合物。相反，微囊藻与短短芽孢杆菌的共培养增强了两种挥发性化合物——β-环柠檬醛和3-甲基-1-丁醇的产生，且前者具有独特的裂解活性。已证实这些挥发性化合物源自蓝藻本身。源自蓝藻的β-紫罗兰酮、土臭素和2-甲基异冰片醇以及植物产生的类似挥发性化合物萜类也具有裂解活性。除少数化合物外，蓝藻代谢产物的最低抑菌浓度值估计高于植物来源的化合物。其中，β-环柠檬醛仅使培养液产生从绿色到蓝色的特征性颜色变化。这种颜色变化类似于在自然环境中蓝藻生长突然下降时观察到的现象。
