Fernandez C, Baune B, Gimenez F, Thuillier A, Farinotti R
Hôpital Pitié Salpétrière, Service Pharmacie, Paris, France.
J Chromatogr. 1991 Dec 6;572(1-2):195-202. doi: 10.1016/0378-4347(91)80483-s.
The enantiomers of zopiclone were determined in human plasma using a sequential achiral-chiral liquid chromatographic method. Zopiclone was separated from the biological matrix and quantified on an achiral silica column. The limit of detection was 5 ng/ml. The eluent fraction containing zopiclone was collected, evaporated, reconstituted with the mobile phase and injected onto a chiral cellulose carbamate column where the enantiomeric ratio was calculated. This validated method, applied to a pilot study, suggests that pharmacokinetics of zopiclone is stereoselective.
采用非手性-手性液相色谱串联法测定人血浆中佐匹克隆的对映体。佐匹克隆从生物基质中分离出来，并在非手性硅胶柱上进行定量分析。检测限为5 ng/ml。收集含有佐匹克隆的洗脱液部分，蒸发、用流动相复溶后注入手性氨基甲酸纤维素柱，计算对映体比例。该验证方法应用于一项初步研究，结果表明佐匹克隆的药代动力学具有立体选择性。
