McCanna David J, Harrington Karen L, Driot Jean-Yves, Ward Keith W, Tchao Ruy
Bausch & Lomb, Rochester, NY 14609, USA.
Eye Contact Lens. 2008 Jan;34(1):6-12. doi: 10.1097/ICL.0b013e31804fa141.
Sodium fluorescein permeability assay, alamarBlue assay, and scanning electron microscopy were performed to study the effects of various contact lens disinfecting multipurpose solutions (MPS) on the integrity of the ocular surface epithelium by using corneal epithelial cells.
The sodium fluorescein permeability and alamarBlue activity of monolayer cultures of human corneal epithelial cells were compared after exposure to ReNu MultiPlus, OPTI-FREE Express, AQuify 5 Minute, SOLO-care Plus With Aqualube, and Complete Moisture Plus contact lens care solutions for 15 minutes. Additional cell monolayers were prepared for each treatment and were analyzed with a scanning electron microscope after a 10-minute exposure.
The sodium fluorescein permeability assay, alamarBlue assay, and scanning electron microscopy showed that OPTI-FREE Express was significantly more damaging to the human corneal epithelial cell monolayer than ReNu MultiPlus, SOLO-care Plus With Aqualube, Complete Moisture Plus, and AQuify 5 Minute contact lens solutions. Cell monolayers treated with OPTI-FREE Express were more permeable to sodium fluorescein and showed lower metabolic activity than cell monolayers treated with the other multipurpose solutions.
This experiment shows that ReNu MultiPlus, SOLO-care Plus With Aqualube, Complete Moisture Plus, and AQuify 5 Minute contact lens solutions have a minimal effect on human corneal epithelial cells in culture, whereas OPTI-FREE Express has a higher negative effect on tight junctions, cell membranes, and overall metabolism of these human corneal epithelial cells.
通过使用角膜上皮细胞，进行荧光素钠通透性测定、alamarBlue测定和扫描电子显微镜检查，以研究各种隐形眼镜消毒护理液（MPS）对眼表上皮完整性的影响。
将人角膜上皮细胞单层培养物暴露于ReNu MultiPlus、OPTI-FREE Express、AQuify 5 Minute、含Aqualube的SOLO-care Plus和Complete Moisture Plus隐形眼镜护理液15分钟后，比较其荧光素钠通透性和alamarBlue活性。为每种处理制备额外的细胞单层，并在暴露10分钟后用扫描电子显微镜进行分析。
荧光素钠通透性测定、alamarBlue测定和扫描电子显微镜检查表明，与ReNu MultiPlus、含Aqualube的SOLO-care Plus、Complete Moisture Plus和AQuify 5 Minute隐形眼镜护理液相比，OPTI-FREE Express对人角膜上皮细胞单层的损伤明显更大。用OPTI-FREE Express处理的细胞单层对荧光素钠的通透性更高，且与用其他护理液处理的细胞单层相比，代谢活性更低。
本实验表明，ReNu MultiPlus、含Aqualube的SOLO-care Plus、Complete Moisture Plus和AQuify 5 Minute隐形眼镜护理液对培养中的人角膜上皮细胞影响最小，而OPTI-FREE Express对这些人角膜上皮细胞的紧密连接、细胞膜和整体代谢具有更高的负面影响。
