Oriowo Mathew Olanrewaju
Department of Optometry and Vision Science, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
Toxicol In Vitro. 2006 Dec;20(8):1548-54. doi: 10.1016/j.tiv.2006.06.013. Epub 2006 Jul 14.
This study investigated the relative ocular lens cytosensitivity to three multipurpose contact lens solutions using lens culture and resazurin microplate assay method. Comparison in the cell viability levels of the cultured bovine lenses as influenced by 3h experimental exposure to three contact lens multipurpose solutions (OPTI-FREE Express, Alcon; ReNu MultiPlus, Bausch & Lomb; and COMPLETE Comfort Plus, Allergan) was performed. The pre- and post-exposure fluorescence emission levels of the lenses were obtained, and findings were compared to baseline and control measurements. The solutions demonstrated varying levels of sensitivity (P<0.1), exhibiting non-significant to significant reversible disruption of the lenses'cell viability levels as revealed by the fluorescence intensity levels in the following ranked order (COMPLETE< ReNu< OPTI-FREE solutions). The findings show that OPTI-FREE Express and ReNu MultiPlus solutions exhibited more cytosensitive effect compared to COMPLETE Comfort Plus. These results suggest that the resazurin assay together with lens culture would be a reliable system for in vitro investigation of the effect of contact lens solutions and other ophthalmic formulations on ocular tissues.
本研究采用晶状体培养和刃天青微孔板检测法，调查了三种多功能隐形眼镜护理液对眼晶状体的相对细胞敏感性。对培养的牛晶状体在实验暴露于三种隐形眼镜多功能护理液（爱尔康公司的OPTI-FREE Express、博士伦公司的ReNu MultiPlus、爱力根公司的COMPLETE Comfort Plus）3小时后，其细胞活力水平进行了比较。获取了晶状体暴露前后的荧光发射水平，并将结果与基线和对照测量值进行比较。这些护理液表现出不同程度的敏感性（P<0.1），从荧光强度水平来看，对晶状体细胞活力水平的可逆性破坏程度从无显著影响到有显著影响，顺序如下（COMPLETE<ReNu<OPTI-FREE护理液）。研究结果表明，与COMPLETE Comfort Plus相比，OPTI-FREE Express和ReNu MultiPlus护理液表现出更强的细胞敏感效应。这些结果表明，刃天青检测法与晶状体培养相结合，将是体外研究隐形眼镜护理液和其他眼科制剂对眼组织影响的可靠系统。
