Jurelevicius Diogo, von der Weid Irene, Korenblum Elisa, Valoni Erika, Penna Mônica, Seldin Lucy
Laboratório de Genética Microbiana, Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, CEP 21941.590 Rio de Janeiro, RJ, Brazil.
J Ind Microbiol Biotechnol. 2008 Apr;35(4):251-5. doi: 10.1007/s10295-007-0297-2. Epub 2008 Jan 8.
Sulfide production by sulfate-reducing bacteria (SRB) is a major concern for the petroleum industry since it is toxic and corrosive, and causes plugging due to the formation of insoluble iron sulfides (reservoir souring). In this study, PCR followed by denaturing gradient gel electrophoresis (PCR-DGGE) using two sets of primers based on the 16S rRNA gene and on the aps gene (adenosine-5-phosphosulfate reductase) was used to track changes in the total bacterial and SRB communities, respectively, present in the water-oil tank system on an offshore platform in Brazil in which nitrate treatment was applied for 2 months (15 nitrate injections). PCR-DGGE analysis of the total bacterial community showed the existence of a dominant population in the water-oil tank, and that the appearance and/or the increase of intensity of some bands in the gels were not permanently affected by the introduction of nitrate. On the other hand, the SRB community was stimulated following nitrate treatment. Moreover, sulfide production did not exceed the permissible exposure limit in the water-oil separation tank studied treated with nitrate. Therefore, controlling sulfide production by treating the produced water tank with nitrate could reduce the quantity of chemical biocides required to control microbial activities.
硫酸盐还原菌（SRB）产生的硫化物是石油工业的一个主要问题，因为它具有毒性和腐蚀性，并且会因不溶性硫化铁的形成（储层酸化）而导致堵塞。在本研究中，使用基于16S rRNA基因和aps基因（腺苷-5-磷酸硫酸还原酶）的两组引物进行聚合酶链反应（PCR），随后进行变性梯度凝胶电泳（PCR-DGGE），分别追踪巴西一个海上平台水油罐系统中存在的总细菌群落和硫酸盐还原菌群落的变化，该系统进行了2个月的硝酸盐处理（15次硝酸盐注入）。对总细菌群落的PCR-DGGE分析表明，水油罐中存在一个优势种群，并且凝胶中一些条带的出现和/或强度增加并未因硝酸盐的引入而受到永久性影响。另一方面，硝酸盐处理后硫酸盐还原菌群落受到刺激。此外，在所研究的用硝酸盐处理的油水分离罐中，硫化物的产生未超过允许暴露极限。因此，通过用硝酸盐处理采出水罐来控制硫化物的产生，可以减少控制微生物活动所需的化学杀菌剂的用量。
