[Change in the function of glucocorticoid receptors and translocation of glucocorticoid receptor complexes in hepatocyte nuclei exposed to nonsteroidal drugs].

Experiments were conducted on male Wistar rats weighing 180-200 g to study the effect of medical agents (Tizercin 3,5.10(-4) M, 7,0.10(-4) M, Cephazolin, streptomycin and penicillin C--10(-3) M, 10(-4) M) on the function of glucocorticoid receptors of hepatic cytosol and translocation of the glucocorticoid-receptor complexes into the nuclei of hepatocytes. Synthetic labeled ligands 3H-dexamethasone and triamcinolone 3H-acetonide were used. Their specific activity was, respectively, 20 and 22 Ci/mmol The association and dissociation constants and the number of glucocorticoid-receptor complexes after their translocation into the hepatocyte nuclei were determined by Sketcher's method. It was found that streptomycin and Tizercin inhibit while Cephazolin and penicillin C activate the function of glucocorticoid receptors. Translocation of glucocorticoid-receptor complexes into the hepatocyte nuclei does not change under the effect of these agents.