Nieman David C, Henson Dru A, Davis J Mark, Dumke Charles L, Gross Sarah J, Jenkins D Paul, Murphy E Angela, Carmichael Martin D, Quindry John C, McAnulty Steven R, McAnulty Lisa S, Utter Alan C, Mayer Eugene P
Department of Biology, Health, Leisure, and Exercise Science, Fischer Hamilton/Nycom Biochemistry Laboratory, Appalachian State University, Boone, North Carolina. 28608, USA.
J Interferon Cytokine Res. 2007 Dec;27(12):1003-11. doi: 10.1089/jir.2007.0050.
The purpose of this study was to measure the influence of quercetin on plasma cytokines, leukocyte cytokine mRNA, and related variables in ultramarathoners competing in the 160-km Western States Endurance Run (WSER). Sixty-three runners were randomized to quercetin and placebo groups and under double-blinded methods ingested 1000 mg/day quercetin for 3 weeks before the WSER. Thirty-nine of the 63 subjects (n = 18 for quercetin, n = 21 for placebo) finished the race and provided blood samples the morning before the race and 15-30 min postrace. Significant prerace to postrace WSER increases were measured for nine proinflammatory and anti-inflammatory plasma cytokines, cortisol (quercetin = 94%, placebo = 96%), serum C-reactive protein (CRP) (mean +/- SE absolute increase, quercetin = 31.8 +/- 4.2, placebo = 38.2 +/- 5.0 mg/L), and creatine kinase (CK) (quercetin = 21,575 +/- 3,977, placebo = 19,455 +/- 3,969 U/L), with no significant group differences. Interleukin-6 (IL-6) mRNA did not change post-WSER, with a significant decrease measured for leukocyte IL-8 mRNA (0.21 +/- 0.03-fold and 0.25 +/- 0.04-fold change from rest, quercetin and placebo, respectively) and significant increases for IL-1Ra mRNA (1.43 +/- 0.18-fold and 1.40 +/- 0.16-fold change, quercetin and placebo, respectively) and IL-10 mRNA (12.9 +/- 3.9-fold and 17.2 +/- 6.1-fold change, quercetin and placebo, respectively), with no significant differences between groups. In conclusion, quercetin ingestion (1 g/day) by ultramarathon athletes for 3 weeks before a competitive 160-km race significantly increased plasma quercetin levels but failed to attenuate muscle damage, inflammation, increases in plasma cytokine and hormone levels, and alterations in leukocyte cytokine mRNA expression.
本研究的目的是测定槲皮素对参加160公里西部各州耐力赛（WSER）的超级马拉松运动员血浆细胞因子、白细胞细胞因子mRNA及相关变量的影响。63名跑步者被随机分为槲皮素组和安慰剂组，并采用双盲法在WSER前3周每天摄入1000毫克槲皮素。63名受试者中的39名（槲皮素组n = 18，安慰剂组n = 21）完成了比赛，并在比赛前一天早晨和赛后15 - 30分钟提供了血样。测定了9种促炎和抗炎血浆细胞因子、皮质醇（槲皮素组 = 94%，安慰剂组 = 96%）、血清C反应蛋白（CRP）（平均±标准误绝对增加值，槲皮素组 = 31.8±4.2，安慰剂组 = 38.2±5.0毫克/升）和肌酸激酶（CK）（槲皮素组 = 21575±3977，安慰剂组 = 19455±3969国际单位/升）从赛前到赛后的显著增加，两组间无显著差异。白细胞介素-6（IL-6）mRNA在WSER后未发生变化，白细胞IL-8 mRNA显著下降（槲皮素组和安慰剂组分别相对于静息时变化0.21±0.03倍和0.25±0.0；4倍），IL-1Ra mRNA和IL-10 mRNA显著增加（槲皮素组和安慰剂组分别变化1.43±0.18倍和1.4±0.16倍；12.9±3.9倍和17.2±6.1倍），两组间无显著差异。总之，超级马拉松运动员在160公里竞争性比赛前3周每天摄入1克槲皮素可显著提高血浆槲皮素水平，但未能减轻肌肉损伤、炎症、血浆细胞因子和激素水平的升高以及白细胞细胞因子mRNA表达的改变。
