Bai Zhigang, Ye Yingjiang, Chen Dingbao, Shen Danhua, Xu Feng, Cui Zhirong, Wang Shan
Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044 Beijing, China.
APMIS. 2007 Dec;115(12):1383-90. doi: 10.1111/j.1600-0463.2007.00654.x.
The aim of the study was to analyze the expression of Cdx2 and nuclear PTEN in relation to clinicopathological features of gastric cancer tissue biopsies in order to determine the value of a combined analysis of Cdx2 and nuclear PTEN expression in distinguishing histological types and prognosis of gastric cancers. The expression of Cdx2 and nuclear PTEN was studied using immunohistochemistry of paraffin-embedded tumor specimens from 99 patients who underwent radical D2 gastrectomy between 1999 and 2001. Cdx2 and nuclear PTEN expression were detected in 39.6% (36 of 91) and 70.3% (64 of 91) of gastric cancer cases, respectively. There was a negative correlation between Cdx2 expression and Lauren classification (p=0.032), and between nuclear PTEN expression and lymph node metastasis (p=0.049). Patients with Cdx2-positive, or nuclear PTEN-positive expression had higher survival rates than those with Cdx2-negative or nuclear PTEN-negative expression (p<0.001 and p=0.003, respectively). Co-expression of Cdx2 and nuclear PTEN showed significantly lower levels in diffuse- or mixed-type cancers than in intestinal-type cancers (p=0.005). Multivariate analysis revealed that Cdx2 expression was an independent prognostic indicator of gastric cancer (p=0.014). These data suggest that combined analysis of Cdx2 and nuclear PTEN expression can have significant value in distinguishing histological types of gastric cancer and assessing prognosis in patients with gastric cancer.
本研究旨在分析Cdx2和细胞核PTEN的表达与胃癌组织活检临床病理特征之间的关系，以确定联合分析Cdx2和细胞核PTEN表达在鉴别胃癌组织学类型和预后方面的价值。使用免疫组织化学方法对1999年至2001年间接受根治性D2胃切除术的99例患者石蜡包埋肿瘤标本中的Cdx2和细胞核PTEN表达进行了研究。在91例胃癌病例中，Cdx2和细胞核PTEN表达的检测率分别为39.6%（91例中的36例）和70.3%（91例中的64例）。Cdx2表达与Lauren分类之间呈负相关（p=0.032），细胞核PTEN表达与淋巴结转移之间呈负相关（p=0.049）。Cdx2阳性或细胞核PTEN阳性表达的患者生存率高于Cdx2阴性或细胞核PTEN阴性表达的患者（分别为p<0.001和p=0.003）。Cdx2和细胞核PTEN的共表达在弥漫型或混合型癌症中的水平显著低于肠型癌症（p=0.005）。多因素分析显示Cdx2表达是胃癌的独立预后指标（p=0.014）。这些数据表明，联合分析Cdx2和细胞核PTEN表达在鉴别胃癌组织学类型和评估胃癌患者预后方面具有重要价值。
