Okayama Hirokazu, Kumamoto Kensuke, Saitou Katsuharu, Hayase Suguru, Kofunato Yasuhide, Sato Yu, Miyamoto Kotaro, Nakamura Izumi, Ohki Shinji, Sekikawa Kouji, Takenoshita Seiichi
Department of Organ Regulatory Surgery, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
Oncol Rep. 2009 Oct;22(4):745-55. doi: 10.3892/or_00000496.
The aim of this study was to examine the expression of CD44v6, CD54, Cdx2, CXCL5, Cyclin B1, MMP-7, nm23, RCAS1 and Survivin in primary gastric cancer and to investigate whether these molecules were useful in predicting the lymph node status. They were selected as candidates for indicators of lymph node metastasis from various kinds of cancer-associated genes reported previously. In 135 cases of radically resected primary gastric adenocarcinoma, we investigated the association between the expression of these molecules and clinocopathologic factors by immunohistochemistry. The results revealed that the expression of CD44v6 and MMP-7 were significantly associated with lymph node status. By contrast, nuclear Cdx2 expression was found to be inversely correlated with lymph node metastasis. Moreover, multivariate analysis demonstrated that CD44v6, MMP-7 and nuclear Cdx2 were independent predictors for lymph node status. In conclusion, our results suggest that positive expression of both CD44v6 and MMP-7, and negative expression of nuclear Cdx2 may serve as powerful predictors of lymph node metastasis in gastric cancer. Combined evaluation of these markers could be further useful to predict lymph node status clinically.
本研究旨在检测原发性胃癌中CD44v6、CD54、Cdx2、CXCL5、细胞周期蛋白B1、基质金属蛋白酶-7（MMP-7）、nm23、逆转录病毒癌基因AS1（RCAS1）和生存素的表达情况，并探究这些分子是否有助于预测淋巴结状态。它们是从先前报道的各种癌症相关基因中筛选出来作为淋巴结转移指标的候选分子。在135例接受根治性切除的原发性胃腺癌患者中，我们采用免疫组织化学方法研究了这些分子的表达与临床病理因素之间的关联。结果显示，CD44v6和MMP-7的表达与淋巴结状态显著相关。相比之下，发现细胞核Cdx2的表达与淋巴结转移呈负相关。此外，多因素分析表明，CD44v6、MMP-7和细胞核Cdx2是淋巴结状态的独立预测因子。总之，我们的结果表明，CD44v6和MMP-7的阳性表达以及细胞核Cdx2的阴性表达可能是胃癌淋巴结转移的有力预测指标。联合评估这些标志物可能在临床上进一步有助于预测淋巴结状态。
