Ostapenko I A, Berman A L, Rychkova M P
Tsitologiia. 1975 Aug;17(8):947-53.
The distribution of NAD kinase and glucose-6-phosphate dehydrogenase within membranes of both outer and inner retina rod segments was studied by the sucrose gradient centrifugation of crude outer segment preparations. Rhodopsin and retinoldehydrogenase served as markers for outer segment membranes, whereas succinate dehydrogenase was a marker for inner ones. It is shown that NAD kinase and glucose-6-phosphate dehydrogenase are localized within inner segment membranes of the photoreception cell and that the activity of these enzymes in the crude preparations is due to contamination of the inner segments.
通过对粗制外段制剂进行蔗糖梯度离心，研究了视杆细胞外段和内段膜中NAD激酶和葡萄糖-6-磷酸脱氢酶的分布。视紫红质和视黄醇脱氢酶作为外段膜的标志物，而琥珀酸脱氢酶作为内段膜的标志物。结果表明，NAD激酶和葡萄糖-6-磷酸脱氢酶定位于光感受器细胞的内段膜中，并且粗制制剂中这些酶的活性是由于内段的污染所致。
