用两种嵌合辛德毕斯病毒/东部马脑炎病毒候选疫苗对骚扰伊蚊和带喙伊蚊进行实验性感染。
Experimental infection of Aedes sollicitans and Aedes taeniorhynchus with two chimeric Sindbis/Eastern equine encephalitis virus vaccine candidates.
作者信息
Arrigo Nicole C, Watts Douglas M, Frolov Ilya, Weaver Scott C
机构信息
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA.
出版信息
Am J Trop Med Hyg. 2008 Jan;78(1):93-7.
Two chimeric vaccine candidates for Eastern equine encephalitis virus (EEEV) were developed by inserting the structural protein genes of either a North American (NA) or South American (SA) EEEV into a Sindbis virus (SINV) backbone. To assess the effect of chimerization on mosquito infectivity, experimental infections of two potential North American bridge vectors of EEEV, Aedes sollicitans and Ae. taeniorhynchus, were attempted. Both species were susceptible to oral infection with all viruses after ingestion of high titer blood meals of ca. 7.0 log(10) plaque-forming units/mL. Dissemination rates for SIN/NAEEEV (0 of 56) and SIN/SAEEEV (1 of 54) were low in Ae. taeniorhynchus and no evidence of transmission potential was observed. In contrast, the chimeras disseminated more efficiently in Ae. sollicitans (19 of 68 and 13 of 57, respectively) and were occasionally detected in the saliva of this species. These results indicate that chimerization of the vaccine candidates reduces infectivity. However, its impact on dissemination and potential transmission is mosquito species-specific.
通过将北美（NA）或南美（SA）东部马脑炎病毒（EEEV）的结构蛋白基因插入辛德毕斯病毒（SINV）骨架中，开发了两种针对EEEV的嵌合疫苗候选物。为了评估嵌合对蚊子感染性的影响，尝试对两种潜在的北美EEEV桥梁媒介——骚扰伊蚊和带喙伊蚊进行实验性感染。在摄入约7.0 log(10) 蚀斑形成单位/毫升的高滴度血餐后，这两个物种对所有病毒的经口感染均敏感。在带喙伊蚊中，SIN/NAEEEV（56只中有0只）和SIN/SAEEEV（54只中有1只）的传播率很低，未观察到传播潜力的证据。相比之下，嵌合体在骚扰伊蚊中传播更有效（分别为68只中有19只和57只中有13只），并且偶尔在该物种的唾液中被检测到。这些结果表明，疫苗候选物的嵌合降低了感染性。然而，其对传播和潜在传播的影响具有蚊子物种特异性。
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