Liu Ji, Min Hang, Ye Lidan
Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou, China.
Biodegradation. 2008 Sep;19(5):695-703. doi: 10.1007/s10532-007-9174-y. Epub 2008 Jan 8.
To assess the co-catabolism of phenanthrene and tricyclazole in different samples, the interaction during the degradation of phenanthrene and tricyclazole were investigated in medium, soil and soil/spent mushroom compost (SMC) mixture. Generally, tricyclazole showed a negative influence on the activity of phenanthrene dioxygenase and it inhibited the degradation of phenanthrene prominently, both in cultures of phenanthrene catabolic isolates (Sphingomonas paucimobilis ZX4 and the mixed flora M1) and soils, while a similar inhibition caused by phenanthrene was also found for the degradation of tricyclazole in soil/SMC. However, the inhibition effect on phenanthrene degradation was eliminated in soil/SMC mixture, due to the abundant microorganisms and enormous catabolic potential in SMC. Furthermore, it was proved that the negative influence between phenanthrene and tricyclazole was most likely derived from the molecular similarity and it tended to decrease with improved microbial diversity in environment.
为评估菲和三环唑在不同样品中的共降解作用，研究了菲和三环唑在培养基、土壤及土壤/菌糠混合物中的降解过程中的相互作用。总体而言，三环唑对菲双加氧酶的活性有负面影响，在菲降解菌（少动鞘氨醇单胞菌ZX4和混合菌群M1）培养物及土壤中均显著抑制菲的降解，而在土壤/菌糠中，菲对三环唑的降解也有类似抑制作用。然而，由于菌糠中丰富的微生物和巨大的分解代谢潜力，在土壤/菌糠混合物中对菲降解的抑制作用被消除。此外，证明了菲和三环唑之间的负面影响很可能源于分子相似性，且随着环境中微生物多样性的提高这种影响趋于降低。
