Osipov V V, Glushankova L N, Toropova F V, Marakhova I I
Tsitologiia. 1991;33(11):94-100.
The measurements were made of monovalent cation (K+ + Rb+, Na+) and protein contents, of leucin incorporation into the protein (protein synthesis), and of ouabain-sensitive K+ influx during the growth of the Jurkat culture cells in the presence or in the absence of ouabain. All the data were calculated per cell. The time dependences of these parameters are nonlinear [correction of unline] curves. The results of three independent measurements are not reproduced. The problem was to find out an intracellular "main" parameter, so that the dependences between this and the rest of parameters be of line character. It has been found that it is the protein synthesis rate that may be taken as the main parameter, because between the protein synthesis rate and all the other parameters there is the line connection. Comparative results obtained with or without ouabain showed that K+ and Na+ concentrations in the cell were different, although the constants of the rate of protein synthesis were the same. This points to the same type of change of the protein synthesis process in the cells growing with or without ouabain.
在有或没有哇巴因存在的情况下，对Jurkat培养细胞生长过程中的单价阳离子（K⁺ + Rb⁺、Na⁺）和蛋白质含量、亮氨酸掺入蛋白质（蛋白质合成）以及哇巴因敏感的K⁺内流进行了测量。所有数据均按每个细胞计算。这些参数的时间依赖性是非线性[纠正“unline”]曲线。三次独立测量的结果无法重现。问题在于找出细胞内的一个“主要”参数，使得该参数与其他参数之间的依赖性呈线性关系。已经发现，蛋白质合成速率可以作为主要参数，因为蛋白质合成速率与所有其他参数之间存在线性联系。有或没有哇巴因时获得的比较结果表明，细胞内的K⁺和Na⁺浓度不同，尽管蛋白质合成速率常数相同。这表明在有或没有哇巴因的情况下生长的细胞中，蛋白质合成过程的变化类型相同。
