Pashinin Iu V, Vinogradova T A, Ignatova T N, Marakhova I I
Tsitologiia. 1988 Mar;30(3):276-82.
Cation fluxes and intracellular content in mouse fibroblasts L, growing for more than three years in the Dulbecco modified Eagle's serum-free medium (clone L625sf) were measured as a function of culture density. The cells show no density-dependent inhibition of growth and in continuously growing cultures of L625sf cells internal potassium, and rubidium influx was found to remain high within a wide range of densities (5.10(4)-20.10(4) cells/cm2). A close correlation was revealed between the potassium transport and the proliferative state of L625sf cultures: the addition of 5% calf serum to logarithmically growing cultures leads to the increase in culture growth rate as well as to the increase in ouabain-inhibited rubidium influx and intracellular potassium content; a delay in culture growth rate due to medium depletion is accompanied by decreasing both the rubidium influx and the intracellular potassium content. It is concluded that L625sf cells being capable of multiplicating in serum-free medium remain sensitive to growth factors of serum and may be used for study of growth factor induction of cell proliferation and for identification of autocrine factors of cell growth.
在杜尔贝科改良伊格尔无血清培养基（克隆L625sf）中生长三年以上的小鼠成纤维细胞L的阳离子通量和细胞内成分，作为培养密度的函数进行了测量。这些细胞未表现出密度依赖性生长抑制，并且在L625sf细胞的连续生长培养物中，发现细胞内钾和铷流入在很宽的密度范围（5×10⁴ - 20×10⁴个细胞/cm²）内保持较高水平。揭示了钾转运与L625sf培养物的增殖状态之间存在密切相关性：向对数生长期培养物中添加5%小牛血清会导致培养物生长速率增加，同时哇巴因抑制的铷流入和细胞内钾含量也增加；由于培养基耗尽导致培养物生长速率延迟，同时铷流入和细胞内钾含量均降低。得出的结论是，能够在无血清培养基中增殖的L625sf细胞对血清生长因子仍敏感，可用于研究生长因子诱导的细胞增殖以及鉴定细胞生长的自分泌因子。
