Marakhova I I, Vinogradova T A, Efimova E N
Tsitologiia. 1987 Feb;29(2):202-7.
Serum stimulation of stationary cultures of Chinese hamster ovary cells CHO-K1 (clone 773) is accompanied by sustained increase in ouabain-sensitive rubidium (potassium) influx which results in the elevation of intracellular potassium content from 0.5-0.6 to 0.7-0.8 mmole per gram of protein. Cytofluorometric studies of serum-stimulated CHO-773 cultures have shown that the intracellular potassium increase is necessary for successful G1----S progression. The elevation of intracellular potassium was found to occur simultaneously with the cellular protein growth. Cycloheximide (10 micrograms/ml) does not influence the early Na,K-ATPase activation induced by serum; however, it abolishes the sustained increase of both rubidium influx and intracellular potassium content. In serum stimulated cells ouabain increases the potassium efflux; this ouabain effect is not observed after S phase, when rubidium (potassium) influx decreases and intracellular potassium content stops growing.
用血清刺激中国仓鼠卵巢细胞CHO-K1（克隆773）的静止培养物时，哇巴因敏感的铷（钾）流入会持续增加，这导致细胞内钾含量从每克蛋白质0.5 - 0.6毫摩尔升高到0.7 - 0.8毫摩尔。对血清刺激的CHO - 773培养物进行的细胞荧光分析表明，细胞内钾的增加是G1期到S期顺利进展所必需的。发现细胞内钾的升高与细胞蛋白质生长同时发生。环己酰亚胺（10微克/毫升）不影响血清诱导的早期钠钾ATP酶激活；然而，它消除了铷流入和细胞内钾含量的持续增加。在血清刺激的细胞中哇巴因会增加钾外流；在S期后未观察到这种哇巴因效应，此时铷（钾）流入减少且细胞内钾含量停止增长。
