使用双光子显微镜对小鼠脊髓中密集分布的神经胶质细胞、轴突和血管进行稳定的体内成像。
Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy.
作者信息
Davalos Dimitrios, Lee Jae K, Smith W Bryan, Brinkman Brendan, Ellisman Mark H, Zheng Binhai, Akassoglou Katerina
机构信息
Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
出版信息
J Neurosci Methods. 2008 Mar 30;169(1):1-7. doi: 10.1016/j.jneumeth.2007.11.011. Epub 2007 Nov 28.
Abstract
In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful tool in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease.
摘要
活体成像彻底改变了我们对大脑生理学和病理学中生物过程的理解。然而，呼吸引起的运动伪影阻碍了这一强大工具在活体脊髓研究中的应用。在此，我们详细描述一种方法，即使用双光子显微镜对具有密集荧光细胞或轴突的小鼠活体脊髓组织进行稳定且重复的成像，无需对动物进行插管或图像后处理。这种简化技术可极大地扩展活体成像在脊髓损伤、再生、生理学和疾病研究中的应用。
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