Schwartz Joel L, Panda Suchismita, Beam Craig, Bach Laura E, Adami Guy R
Department of Oral Medicine Diagnostic Sciences, Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60610, USA.
J Oral Pathol Med. 2008 Feb;37(2):70-7. doi: 10.1111/j.1600-0714.2007.00596.x.
RNA expression analysis of oral keratinocytes can be used to detect early stages of disease such as oral cancer or to monitor on-going treatment responses of the same or other oral diseases. A limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression.
As a starting point in the analysis of tumor markers in oral squamous cell carcinoma (OSCC), we examined RNA in brush cytology samples from hamsters treated with dibenzo[a,l]pyrene to induce oral carcinoma. Three separate samples from each animal were assessed for expression of candidate marker genes and control genes measured with real-time RT-PCR.
Brush oral cytology samples from normal mucosa were shown to consist almost exclusively of epithelial cells. Remarkably, ss-2 microglobulin and cytochrome p450, 1B1 (CYP1B1) RNA showed potential utility as markers of OSCC in samples obtained in this rapid and non-surgical manner.
Brush oral cytology may prove useful as a source of RNA for gene expression analysis during the progression of diseases of the oral epithelium such as OSCC.
口腔角质形成细胞的RNA表达分析可用于检测疾病的早期阶段，如口腔癌，或监测同一或其他口腔疾病正在进行的治疗反应。一个局限性是在不进行活检的情况下无法从口腔组织中获得高质量的RNA。虽然口腔细胞学细胞样本可以以微创方式从患者身上获取，但它们尚未经过验证用于RNA表达的定量分析。
作为分析口腔鳞状细胞癌（OSCC）肿瘤标志物的起点，我们检测了用二苯并[a,l]芘诱导口腔癌的仓鼠刷状细胞学样本中的RNA。用实时RT-PCR对每只动物的三个独立样本进行候选标记基因和对照基因表达的评估。
正常黏膜的刷状口腔细胞学样本几乎完全由上皮细胞组成。值得注意的是，在以这种快速且非手术方式获得的样本中，β2-微球蛋白和细胞色素P450 1B1（CYP1B1）RNA显示出作为OSCC标志物的潜在效用。
刷状口腔细胞学可能被证明是口腔上皮疾病（如OSCC）进展过程中用于基因表达分析的RNA来源。
