Kristinsson Jón O, van Westerveld Paul, te Morsche Rene H M, Roelofs Hennie M J, Wobbes T, Witteman Ben J M, Tan Adriaan C I T L, van Oijen Martijn G H, Jansen Jan B M J, Peters Wilbert H M
Department of Gastroenterology, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
World J Gastroenterol. 2009 Jul 28;15(28):3493-7. doi: 10.3748/wjg.15.3493.
To determine whether -1195 A-->G and/or -765 G-->C polymorphisms in Cyclooxygenase-2 (COX-2) may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population.
Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios (OR) and 95% confidence intervals (CI) were estimated.
The distribution of the -1195 A-->G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma (OR = 3.85, 95% CI: 1.45-10.3) compared with the -1195 AA genotype as a reference. The -765 G-->C genotype distribution was not different between the two groups. The GG/GG haplotype was present more often in esophageal adenocarcinoma patients than in controls (OR = 3.45, 95% CI: 1.24-9.58; with AG/AG as a reference). The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance.
Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma.
确定环氧化酶-2（COX-2）基因中-1195 A→G和/或-765 G→C多态性是否会对荷兰白种人群食管癌的发生产生风险修饰作用。
招募了两个研究组，252例食管癌患者和240名健康对照，两组在种族、年龄、性别和招募地区方面相匹配。从全血中分离DNA并用于基因分型。PCR产物用限制性内切酶消化，产物通过琼脂糖凝胶电泳进行分析。估计优势比（OR）和95%置信区间（CI）。
与对照组相比，食管癌患者中-1195 A→G多态性的分布有显著差异。以-1195 AA基因型为参照，-1195 GG基因型导致发生食管腺癌的风险更高（OR = 3.85，95% CI：1.45 - 10.3）。两组之间-765 G→C基因型分布无差异。食管腺癌患者中GG/GG单倍型出现的频率高于对照组（OR = 3.45，95% CI：1.24 - 9.58；以AG/AG为参照）。在鳞状细胞癌患者中也观察到相同趋势，然而，结果未达到统计学显著性。
COX-2 -1195 GG基因型和GG/GG单倍型的存在可能导致发生食管癌的风险更高。
