Department of Digestive Diseases, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing, 100050, China.
Pathol Oncol Res. 2010 Mar;16(1):39-45. doi: 10.1007/s12253-009-9185-6. Epub 2009 Jul 25.
Cyclooxygenase-2 (COX-2) has been shown to be upregulated in a variety of tumors so that COX-2 may be a potential target in the treatment of cancer. In order to further explore the mechanism, we used RNA interference to study effects of the inhibition of COX-2 on esophageal squamous cell carcinoma (ESCC) lines. Western blot analysis demonstrated that COX-2 expression was significantly reduced in ESCC cells treated with the COX-2-specific siRNA. Furthermore, the COX-2 siRNA treatment inhibited cell proliferation and induced apoptosis in ESCC cells. In addition, the combination treatment of COX-2 siRNA and acidum acetil salicylicum (aspirin) has a synergistic effect. Therefore, this combination has potential as an anticancer therapy for the treatment of ESCC.
环氧化酶-2（COX-2）在多种肿瘤中呈上调表达，因此 COX-2 可能成为癌症治疗的潜在靶点。为了进一步探讨其机制，我们采用 RNA 干扰技术研究抑制 COX-2 对食管鳞癌细胞系的影响。Western blot 分析表明 COX-2 特异性 siRNA 处理可显著降低 ESCC 细胞中 COX-2 的表达。此外，COX-2 siRNA 处理抑制 ESCC 细胞增殖并诱导其凋亡。此外，COX-2 siRNA 和乙酰水杨酸（阿司匹林）联合治疗具有协同作用。因此，这种联合治疗具有作为治疗 ESCC 的抗癌疗法的潜力。
