Gansuvd Balgansuren, Goodwin Jeanine, Asiedu Clement K, Jiang Xiao Ling, Jargal Uuganbayar, Andrades Patricio, Exley Mark A, Thomas Judith M
Department of Surgery, University of Alabama at Birmingham, 1670 University Boulevard, Birmingham, AL 35294, USA.
J Med Primatol. 2008 Feb;37(1):1-11. doi: 10.1111/j.1600-0684.2007.00222.x.
Natural killer T cells (NKT) possess dual functions of innate and adaptive immune systems, controlling viral infections and regulating autoimmune diseases. Non-human primates (NHP) are penultimate models for advancing therapeutic immunoregulatory strategies for translational application in humans, though, little is known about NHP NKT cells. Here we characterized rhesus macaque NKT cells ex vivo.
The frequency, phenotype and intracellular cytokine production of V alpha 24+ 6B11+ invariant NKT (iNKT) cells were analyzed by multi-color flow cytometry. V alpha 24J alpha Q mRNA expression was analyzed by real-time RT-PCR.
The frequencies of peripheral blood (PB) and spleen V alpha 24+ 6B11+ iNKT cells were not significantly different. The iNKT cell subset in spleen was significantly increased for CD4+ CD8+ and CD3+ CD56+ co-expression as well as intracellular interleukin-4 production, which was rarely observed in circulating PB.
Spleen iNKT cells in rhesus macaques are Th2 biased and display phenotypically and functionally distinct profiles from their PB counterpart.
自然杀伤T细胞（NKT）具有先天性和适应性免疫系统的双重功能，可控制病毒感染并调节自身免疫性疾病。非人灵长类动物（NHP）是推进治疗性免疫调节策略以用于人类转化应用的理想模型，然而，关于NHP的NKT细胞却知之甚少。在此，我们对恒河猴NKT细胞进行了体外特征分析。
通过多色流式细胞术分析Vα24 + 6B11 +不变自然杀伤T细胞（iNKT）的频率、表型和细胞内细胞因子的产生。通过实时逆转录聚合酶链反应分析Vα24JαQ mRNA表达。
外周血（PB）和脾脏中Vα24 + 6B11 + iNKT细胞的频率无显著差异。脾脏中的iNKT细胞亚群在共表达CD4 + CD8 +和CD3 + CD56 +以及细胞内白细胞介素-4产生方面显著增加，而在循环外周血中很少观察到这种情况。
恒河猴脾脏中的iNKT细胞偏向于Th2型，并且在表型和功能上与其外周血对应物具有不同的特征。
