工业蛋白酶“碱性蛋白酶”在肽合成中的应用。
Application of industrial protease "Alcalase" in peptide synthesis.
作者信息
Chen S T, Chen S Y, Hsiao S C, Wang K T
机构信息
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
出版信息
Biomed Biochim Acta. 1991;50(10-11):S181-6.
An industrial alkaline protease "Alcalase" has been found to be very stable in organic solvents and usable as a catalyst for i) resolution of N-protected amino acids, in both aqueous solution and organic solvent with high yield and optical purity, ii) saponification of Fmoc-peptide esters in high concentration organic solvent iii) kinetically controlled peptide bond formation in ethanol solution, iv) diastereoselective hydrolysis of peptide esters, to prepare optically pure peptides.
一种工业碱性蛋白酶“碱性蛋白酶”已被发现，在有机溶剂中非常稳定，可用作以下反应的催化剂：i）在水溶液和有机溶剂中拆分N-保护氨基酸，产率高且光学纯度高；ii）在高浓度有机溶剂中皂化Fmoc-肽酯；iii）在乙醇溶液中动力学控制肽键形成；iv）肽酯的非对映选择性水解，以制备光学纯肽。
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