Heiduschka P, Dittrich J, Heins J, Neubert K, Barth A
Pädagogische Hochschule N.K. Krupskaja, Halle-Köthen, Martin-Luther-Universität Halle-Wittenberg, Biotechnikum.
Pharmazie. 1989 Nov;44(11):778-80.
According to the increasing importance of enzyme catalysed peptide synthesis the capability of the protease dipeptidyl peptidase IV is shown to form the peptide bond. To our best knowledge, it's the first time that has been used successfully proline as one of the amino acids. The formation of the peptide bond is kinetically controlled. Free amino acids have been used as nucleophiles. The reaction media contain 50 vol.-% glycerol. A first approach to a kinetic treatment is shown.
随着酶催化肽合成的重要性日益增加，蛋白酶二肽基肽酶IV形成肽键的能力得到了展示。据我们所知，脯氨酸首次成功地被用作氨基酸之一。肽键的形成受动力学控制。游离氨基酸被用作亲核试剂。反应介质含有50体积%的甘油。展示了动力学处理的初步方法。
